Effects of light quality on accumulation of phenolic compounds and antioxidant activities in Cyclocarya paliurus (Batal.) Iljinskaja leaves from different families

doi:10.3969/j.issn.1000-2006.201901015

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2020, Vol. 44 ›› Issue (2): 17-25.doi: 10.3969/j.issn.1000-2006.201901015

Special Issue: 青钱柳叶有效成分专题

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Effects of light quality on accumulation of phenolic compounds and antioxidant activities in Cyclocarya paliurus (Batal.) Iljinskaja leaves from different families

CHEN Pei¹(), ZHOU Mingming¹, FANG Shengzuo¹^,²^,^*(), LIU Yang¹, YANG Wanxia¹^,², SHANG Xulan¹^,²

1. College of Forestry, Nanjing Forestry University, Nanjing 210037, China
2. Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China

Received:2019-01-10 Revised:2019-05-25 Online:2020-03-30 Published:2020-04-01
Contact: FANG Shengzuo E-mail:1292025985@qq.com;fangsz@njfu.edu.cn

Abstract

Abstract:

【Objective】Light quality has a certain effect on the accumulation of phenolic compounds in plants. Cyclocarya paliurus (Batal.) Iljinskaja is a unique species in China, it is widely used as a material for wood, medicines and ornamentation. The objective of the present study was to investigate the effect of light quality on the accumulation of phenolic compounds in C. paliurus (Batal.) Iljinskaja leaves from different families and their antioxidant activity and thereby provide a basis for optimizing the light environment during seedling development and further development ofC. paliurus (Batal.) Iljinskaja healthcare products.【Method】Current-year seedlings of C. paliurus (Batal.) Iljinskaja from four families (Guangxi Jinzhongshan No. 7, Guangxi Jinzhongshan No. 6, Sichuan Muchuan No. 31 and Zhejiang Anji No. 1) were treated with four light quality groups (white light, blue light, green light and red light) with controlled temperature and humidity in an artificial climate chamber. Leaf biomass, phenolic content and yield in C. paliurus (Batal.) Iljinskaja leaves as well as phenolic antioxidant activity [DPPH (1, 1-diphenyl-2-picrylhydrazyl) radical-scavenging ability and FRAP (ferric reducing power assay)]in vitro were measured, and the correlation between phenolic content and antioxidant activity was analyzed.【Result】Light quality and family and their interaction had significant effects on leaf biomass, phenolic content and yield per plant and antioxidant activity. However, there were no significant effects of family on the total flavonoid content and yield per plant. In general, white light treatment resulted in the highest value of leaf biomass but the lowest value of phenolic content and yield per plant and antioxidant activity. The seedlings treated with blue light and green light showed higher content of phenolics and antioxidant activity, but green light treatment resulted in the lowest value of leaf biomass. Therefore, blue light treatment was more beneficial in improving the accumulation of phenolics and their antioxidant capacity inC. paliurus (Batal.) Iljinskaja leaves. At the family’s level, the leaf biomass ofC. paliurus (Batal.) Iljinskaja from Anji No. 1 was the highest. However, the highest values of phenolic content and yield per plant and their antioxidant capacity were observed in Jinzhongshan No. 6. The correlation analysis showed that the content of total phenolics and phenolic acids significantly correlated with the antioxidant activity ( P < 0.05). 【Conclusion】Regulation of light quality significantly affected the accumulation of phenolics and their antioxidant activity in the leaves of C. paliurus (Batal.) Iljinskaja belonging to the different families. Leaves of C. paliurus (Batal.) Iljinskaja from Jinzhongshan No. 6 treated with blue light accumulated more phenolics and thereby exhibited higher antioxidant ability. The results provide a reference for the development of C. paliurus (Batal.) Iljinskaja healthcare products and light management for directional cultivation in C. paliurus (Batal.) Iljinskaja plantations.

Key words: Cyclocarya paliurus (Batal.) Iljinskaja, light quality, family, phenolics, antioxidant activities, blue light

CLC Number:

S792.99
S718

CHEN Pei, ZHOU Mingming, FANG Shengzuo, LIU Yang, YANG Wanxia, SHANG Xulan. Effects of light quality on accumulation of phenolic compounds and antioxidant activities in Cyclocarya paliurus (Batal.) Iljinskaja leaves from different families[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2020, 44(2): 17-25.

Figures/Tables 6

Table 1

Fig.1

Fig.2

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Fig.4

Table 2

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处理号 treatment No.	光质 light quality	家系 family
WJ7	WL(白色LED)	J7(金钟山7号)
WJ6	WL(白色LED)	J6(金钟山6号)
WM	WL(白色LED)	M(沐川31号)
WA	WL(白色LED)	A(安吉1号)
BJ7	BL(蓝色LED)	J7(金钟山7号)
BJ6	BL(蓝色LED)	J6(金钟山6号)
BM	BL(蓝色LED)	M(沐川31号)
BA	BL(蓝色LED)	A(安吉1号)
GJ7	GL(绿色LED)	J7(金钟山7号)
GJ6	GL(绿色LED)	J6(金钟山6号)
GM	GL(绿色LED)	M(沐川31号)
GA	GL(绿色LED)	A(安吉1号)
RJ7	RL(红色LED)	J7(金钟山7号)
RJ6	RL(红色LED)	J6(金钟山6号)
RM	RL(红色LED)	M(沐川31号)
RA	RL(红色LED)	A(安吉1号)

抗氧化活性 antioxidant activity	EC₅₀	TPC	TF	TPA	PA1	PA2	PA3	F1	F2	F3	F4	F5	F6
IC₅₀	0.810^**	-0.653^**	-0.340	-0.785^**	-0.730^**	-0.773^**	-0.706^**	-0.306	-0.354	-0.325	-0.252	-0.326	-0.265
EC₅₀	—	-0.584^*	-0.195	-0.799^**	-0.768^**	-0.780^**	-0.661^**	-0.178	-0.195	-0.225	-0.131	-0.185	-0.155

Effects of light quality on accumulation of phenolic compounds and antioxidant activities in Cyclocarya paliurus (Batal.) Iljinskaja leaves from different families

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