JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2020, Vol. 44 ›› Issue (3): 49-57.doi: 10.3969/j.issn.1000-2006.201904039
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YANG Ying(), DUAN Hao, GUO Jinbo, WANG Ziyang, SHI Qin, XUAN Lei, YU Chaoguang(
)
Received:
2019-04-03
Revised:
2020-01-13
Online:
2020-05-30
Published:
2020-06-11
Contact:
YU Chaoguang
E-mail:yingyang@cnbg.net;527884385@qq.com
CLC Number:
YANG Ying, DUAN Hao, GUO Jinbo, WANG Ziyang, SHI Qin, XUAN Lei, YU Chaoguang. Genetic variation and QTL analysis of rooting traits of backcross progenies of(Taxodium distichum×T. mucronatum)×T. mucronatum hardwood cuttings steckling[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2020, 44(3): 49-57.
Table 1
Phenotypic eigenvalues of rooting?related traits of (T. distichum×T. mucronatum) ×T. mucronatum BC1 hybrids"
性状 traits | 生根率/%rooting rate | 发根数 number of adventitious roots | 最大根粗/cm the diameter of the largest root | 根长/cm root length | 抽梢长/cm shoot length | 抽梢地径/cm shoot diameter | |||
---|---|---|---|---|---|---|---|---|---|
最长根 the longest root | 次长根 the second root | 第3根 the third root | 平均 mean length of the first to three root | ||||||
变异系数/% CV | 54.61 | 31.14 | 37.92 | 31.24 | 35.24 | 38.76 | 32.64 | 63.67 | 35.65 |
最大值max. | 0.73 | 11.45 | 0.64 | 21.00 | 18.81 | 12.28 | 17.06 | 1.89 | 39.45 |
最小值min. | 0.03 | 2.00 | 0.09 | 2.50 | 2.00 | 0.00 | 2.00 | 0.09 | 4.70 |
平均值mean | 0.28 | 6.21 | 0.24 | 11.03 | 8.13 | 6.41 | 8.53 | 0.27 | 18.15 |
标准差SD | 0.16 | 1.93 | 0.09 | 3.45 | 2.87 | 2.49 | 2.78 | 0.17 | 6.47 |
Table 2
Phenotypic correlation analysis between rooting?related traits of (T. distichum×T. mucronatum) ×T. mucronatum BC1 hybrids"
性状 traits | 生根率 rooting rate | 发根数 number of adventitious roots | 最大根粗 diameter of the largest root | 最长根长 the longest root length | 次长根长 the second root length | 第3根长 the third root length | 平均根长 mean length of the first to three roots | 抽梢地径 shoot diameter |
---|---|---|---|---|---|---|---|---|
发根数number of adventitious roots | -0.142 | |||||||
最大根粗diameter of the largest root | -0.103 | -0.084 | ||||||
最长根长the longest root length | 0.147 | 0.002 | 0.575** | |||||
次长根长the second root length | 0.071 | 0.107 | 0.502** | 0.860** | ||||
第3根长the third root length | 0.072 | 0.266** | 0.437** | 0.797** | 0.900** | |||
平均根长mean length of the first to three roots | 0.107 | 0.116 | 0.539** | 0.945** | 0.966** | 0.935** | ||
抽梢地径shoot diameter | 0.106 | 0.024 | 0.193* | 0.360** | 0.302** | 0.284** | 0.337** | |
抽梢长度shoot length | 0.016 | 0.097 | 0.552** | 0.760** | 0.791** | 0.772** | 0.815** | 0.377** |
Table 3
Characterization of major QTLs for rooting?related traits"
名称 name | 连锁群 linkage group | 标记区间 marker interval | 标记数目 No. | 物理图距间隔/cm physical distance interval | 物理图距/ cm length | 性状 traits | 解释表型变异率/% phenotypic variance explained(PVE) |
---|---|---|---|---|---|---|---|
q3-1 | 3 | 4574~20672 | 3 | 25.64~26.303 | 0.663 | 平均根长 | 10.73 |
第3根长 | 12.78 | ||||||
q3-2 | 3 | 63134~63134 | 1 | 56.488~56.488 | 0 | 生根率 | 5.07 |
q4-1 | 4 | 28255~87168 | 30 | 0.647~0.647 | 0 | 发根数 | 8.72 |
q8-1 | 8 | 9233~87586 | 48 | 41.149~43.933 | 2.784 | 平均根长 | 4.24 |
第3根长 | 4.53 | ||||||
q9-1 | 9 | 73370~57140 | 12 | 10.041~10.041 | 0 | 平均根长 | 6.67 |
最长根长 | 6.37 | ||||||
次长根长 | 6.91 | ||||||
第3根长 | 4.80 | ||||||
q10-1 | 10 | 84935~17501 | 4 | 46.176~46.176 | 0 | 生根率 | 12.05 |
q11-1 | 11 | 7574~55445 | 4 | 90.176~90.176 | 0 | 抽梢地径 | 6.68 |
Table 4
Function prediction of SLAF markers in major QTL intervals"
QTL | 标记号 marker ID | unigene ID | E | Nr功能注释 Nr annotation |
---|---|---|---|---|
q3-1 | 20672 | CL1509.contig4_All | 2×10-33 | 转座子TNT 1中逆转录病毒相关的Pol多聚蛋白retrovirus?related Pol polyprotein from transposon TNT 1 |
q3-2 | 63134 | CL1070.contig14_All | 2×10-11 | ABC转运蛋白B家庭成员11 ABC transporter B family member 11 |
q4-1 | 73435 | CL576.contig29_All | 5×10-28 | 含有mTERF结构域蛋白,线粒体mTERF domain?containing protein, mitochondrial |
16943 | CL2046.contig8_All | 1×10-22 | GDSL酯酶/脂肪酶GDSL esterase/lipase | |
52454 | CL2377.contig2_All | 2×10-30 | 泛素结合酶E2Q样蛋白1 OSUbiquitin?conjugating enzyme E2Q?like protein 1 | |
7163 | CL5658.contig1_All | 2×10-12 | 转座子TNT 1中逆转录病毒相关的Pol多聚蛋白retrovirus?related Pol polyprotein from transposon TNT 1 | |
171375 | unigene7476_All | 1×10-16 | 富含亮氨酸的重复延伸蛋白样蛋白leucine?rich repeat extensin?like protein | |
67290 | unigene7428_All | 1×10-43 | 富含亮氨酸的重复蛋白SHOC2leucine?rich repeat protein SHOC2 | |
32131 | CL1045.contig6_All | 2×10-14 | 含冷激结构域蛋白3cold shock domain?containing protein 3 | |
8383 | CL1509.contig4_All | 2×10-27 | 转座子TNT 1中逆转录病毒相关的Pol多聚蛋白retrovirus?related Pol polyprotein from transposon TNT 1 | |
18792 | comp47665_c0 | 8×10-22 | 光系统II CP47蛋白(psbB)基因photosystem II CP47 protein (psbB) gene | |
51035 | comp26301_c0 | 1×10-17 | 单加氧酶monooxygenase | |
37850 | comp936956_c0 | 4×10-39 | 磷酸酶IIphosphatases II | |
82602 | unigene41006_All | 4×10-16 | 转座子TNT 1中逆转录病毒相关的Pol多聚蛋白retrovirus?related Pol polyprotein from transposon TNT 1 | |
q9-1 | 38681 | CL911.contig17_All | 2×10-11 | 小核糖核蛋白sm d1Small nuclear ribonucleo protein sm d1 |
29130 | CL2431.contig4_All | 4×10-50 | 转录起始因子TFIID亚基6异构体 X2transcription initiation factor TFIID subunit 6 isoform X2 | |
9283 | unigene40412_All | 1×10-19 | 含G?patch结构域蛋白 G?patch domain?containing protein | |
16964 | unigene6644_All | 1×10-25 | U3小核仁RNA(U3 snorna)相关蛋白U3 small nucleolar RNA (U3 snorna) associated protein |
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