EAG and olfactory behavioral responses of Anoplophora glabripennis (Coleoptera: Cerambycidae) to volatiles of Acer saccharum

doi:10.12302/j.issn.1000-2006.201907048

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2021, Vol. 45 ›› Issue (1): 123-130.doi: 10.12302/j.issn.1000-2006.201907048

Previous Articles Next Articles

EAG and olfactory behavioral responses of Anoplophora glabripennis (Coleoptera: Cerambycidae) to volatiles of Acer saccharum

MA Xiaoqian¹^,²(), GE Jun², WANG Qi¹, ZHAO Hongying¹, SUN Yan¹, GAO Yu¹, SHEN Guotao¹, YU Wenxi¹

1. Institute of Forest Protection, Heilongjiang Academy of Forestry, Harbin 150081,China
2. College of Forestry, Northeast Forestry University, Harbin 150040, China

Received:2019-07-31 Accepted:2020-04-08 Online:2021-01-30 Published:2021-02-01

Abstract

Abstract:

【Objective】 This study was conducted to identify the volatile components of Acer saccharum, explore how the volatile constituents of Acer saccharum affect the host position of Anoplophora glabripennis, analyze the role of host volatiles in the search for and host positioning of Anoplophora glabripennis, and provide new ideas and a theoretical basis for the comprehensive prevention and control of Anoplophora glabripennis infestations. 【Method】The volatile components of Acer saccharum were identified by TCT-GC-MS. The electrophysiological and behavioral responses of Anoplophora glabripennis to Acer saccharum volatiles were determined by the electroantennogram (EAG) and Y-tube olfactometer. The main volatiles affecting the search and host location of A. glabripennis were identified by multiple comparison methods, an independent sample t test, and a chi-square test analysis. 【Result】The volatiles released from Acer saccharum included 34 volatile compounds, the main components of which were esters (61.67%), alkenes (12.99%), acids (5.58%) and alcohols (5.02%), respectively. There were electrophysiological and behavioral responses to 12 volatile compounds. The electrophysiological and behavioral responses of the female and male A. glabripennis adults were significantly different to the same concentration of different volatiles, the same volatiles, and different concentrations of the same volatiles. Male and female A. glabripennis adults exhibited obvious electrophysiological responses to hexyl acetate, diisobutyl adipate, diisobutyl phthalate, hexenyl acetate, geranyl propionate, cis-3-hexenol, 2-hexyl-1-decanol, ocimene and squalene. Among these, the female and male adults of A. glabripennis showed a significant attraction to hexyl acetate, hexenyl acetater, geranyl propionate, cis-3-hexenol, and 2-hexyl-1-nonanol, while ocimene and squalene had significant repellent effects. Female adults of A. glabripennis were significantly attracted to diisobutyl adipate and lauric acid, but these compounds elicited no behavioral response from male adults. 【Conclusion】 Female and male adults of A. glabripennis are strongly attracted to hexenyl acetater, geranyl propionate, cis-3-hexenol, and 2-hexyl-1-nonanol, while ocimene and squalene show good repellent qualities. Diisobutyl adipate and lauric acid were significantly attractive to female adults of A. glabripennis, but male adults showed no behavioral response.

Key words: Anoplophora glabripennis, Acer saccharum, volatiles, TCT-GC-MS, EAG, olfactory behavior

CLC Number:

Q968
S763

MA Xiaoqian, GE Jun, WANG Qi, ZHAO Hongying, SUN Yan, GAO Yu, SHEN Guotao, YU Wenxi. EAG and olfactory behavioral responses of Anoplophora glabripennis (Coleoptera: Cerambycidae) to volatiles of Acer saccharum[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2021, 45(1): 123-130.

Figures/Tables 3

Table 1

Table 2

EAG responses of female and male Anoplophora glabripennis adult to 12 volatiles of Acer saccharum"

化合物 compound	浓度/ (mol·L^-1) concentration	EAG反应值/mV EAG		P	化合物 compound	浓度/ (mol·L^-1) concentration	EAG反应值/mV EAG				P
化合物 compound	浓度/ (mol·L^-1) concentration	雌虫 female	雄虫 male	P	化合物 compound	浓度/ (mol·L^-1) concentration	雌虫 female		雄虫 male		P
液态石蜡	CK	0.086±0.001 a	0.086±0.000 a	1.000	液态石蜡	CK		0.086±0.001 a		0.086±0.000 a		1.000
乙酸己酯	0.000 4	0.101±0.001 b	0.092±0.002 b	0.034^*	十四酸	0.000 4		0.068±0.002 a		0.073±0.003 a		0.185
	0.004 0	0.145±0.001 c	0.113±0.001 c	0.000^**		0.004 0		0.064±0.002 a		0.081±0.002 a		0.004^**
	0.040 0	0.182±0.002 d	0.147±0.000 d	0.000^**		0.040 0		0.084±0.003 a		0.092±0.001 b		0.046^*
	0.400 0	0.261±0.002 e	0.225±0.001 e	0.000^**		0.400 0		0.170±0.000 b		0.143±0.001 c		0.000^**
	2.000 0	0.348±0.005 f	0.296±0.001 f	0.007^**		2.000 0		0.200±0.010 c		0.124±0.002 b		0.016^*
己二酸二异丁酯	0.000 4	0.085±0.002 a	0.078±0.001 a	0.022^*	月桂酸	0.000 4		0.085±0.001 a		0.083±0.002 a		1.000
	0.004 0	0.100±0.002 a	0.088±0.002 a	0.016^*		0.004 0		0.091±0.001 b		0.088±0.002 a		0.275
	0.040 0	0.134±0.000 b	0.081±0.002 a	0.001^**		0.040 0		0.128±0.001 c		0.098±0.002 b		0.000^**
	0.400 0	0.175±0.001 c	0.089±0.002 ab	0.000^**		0.400 0		0.214±0.001 d		0.119±0.002 c		0.000^**
	2.000 0	0.248±0.008 d	0.105±0.004 b	0.001^**		2.000 0		0.240±0.000 e		0.144±0.002 d		0.000^**
邻苯二甲酸二异丁酯	0.000 4	0.087±0.001 a	0.085±0.002 a	0.441	罗勒烯	0.000 4		0.086±0.003 a		0.087±0.002 a		0.636
	0.004 0	0.103±0.001 b	0.099±0.001 b	0.078		0.004 0		0.084±0.002 a		0.086±0.000 a		0.135
	0.040 0	0.123±0.001 c	0.115±0.001 b	0.045^*		0.040 0		0.143±0.003 b		0.118±0.002 b		0.022^*
	0.400 0	0.144±0.000 d	0.121±0.001 c	0.020^*		0.400 0		0.228±0.002 c		0.167±0.001 c		0.000^**
	2	0.223±0.003 e	0.189±0.002 d	0.000^**		2		0.314±0.001 d		0.247±0.002 d		0.000^**
棕榈酸甲酯	0.000 4	0.082±0.002 a	0.081±0.001 a	0.701	角鲨烯	0.000 4		0.075±0.002 a		0.075±0.001 a		1.000
	0.004 0	0.089±0.003 a	0.100±0.004 b	0.013^*		0.004 0		0.068±0.003 a		0.094±0.002 b		0.002^**
	0.040 0	0.115±0.001 b	0.121±0.001 c	0.086		0.040 0		0.107±0.001 a		0.107±0.001 c		1.000
	0.400 0	0.128±0.001 c	0.185±0.002 d	0.000^**		0.400 0		0.141±0.001 b		0.148±0.005 c		0.042^*
	2.000 0	0.187±0.002 d	0.259±0.002 e	0.000^**		2.000 0		0.154±0.003 b		0.167±0.002 d		0.000^**
乙酸叶醇酯	0.000 4	0.120±0.012 b	0.116±0.001 b	0.191	顺-3- 己烯醇	0.000 4		0.110±0.001 b		0.102±0.002 b		0.046^*
	0.004 0	0.236±0.012 c	0.127±0.000 c	0.000^**		0.004 0		0.127±0.001 c		0.109±0.002 b		0.002^**
	0.040 0	0.347±0.001 d	0.147±0.001 d	0.000^**		0.040 0		0.271±0.001 d		0.245±0.001 c		0.000^**
	0.400 0	0.437±0.002 e	0.239±0.002 e	0.000^**		0.400 0		0.351±0.001 e		0.334±0.001 d		0.000^**
	2.000 0	0.554±0.003 f	0.327±0.001 f	0.000^**		2.000 0		0.449±0.001 f		0.412±0.003 e		0.000^**
丙酸叶醇酯	0.000 4	0.097±0.004 b	0.089±0.001 a	0.053	2-己基- 1-癸醇	0.000 4		0.083±0.002 a		0.082±0.002 a		1.000
	0.004 0	0.112±0.001 b	0.103±0.001 ab	0.025^*		0.004 0		0.081±0.002 a		0.084±0.002 a		0.189
	0.040 0	0.175±0.000 c	0.128±0.001 c	0.000^**		0.040 0		0.094±0.003 b		0.110±0.001 b		0.002^**
	0.400 0	0.262±0.001 d	0.161±0.002 d	0.000^**		0.400 0		0.102±0.001 b		0.114±0.001 b		0.001^**
	2.000 0	0.340±0.001 e	0.256±0.002 e	0.000^**		2.000 0		0.220±0.021 c		0.161±0.002 c		0.000^**

Table 2

Table 3

Olfactory behavioral responses of female and male Anoplophora glabripennis adult to twelve volatiles of Acer saccharum"

挥发性化合物 compound		浓度/ (mol·L^-1) concentration		引诱率/% lure rate				驱避率/% repellent rate				P			挥发性化合物 compound		浓度/ (mol·L^-1) concentration										引诱率/% lure rate						驱避率/% repellent rate			P
挥发性化合物 compound		浓度/ (mol·L^-1) concentration		雌 female		雄 male		雌 female		雄 male		雌 female		雄 male	挥发性化合物 compound		浓度/ (mol·L^-1) concentration										雌 female		雄 male		雌 female		雄 male		雌 female	雄 male
乙酸己酯		0.000 4		20 aB		12 aA		16		12		0.505		1.000	十四酸		0.000 4		8 aA		8 aA			12	8 aA		0.371								1.000
		0.004 0		28 bB		24 bB		20		20		0.248		0.546			0.004 0		24 bB		20bA			24	20 bA		1.000								1.000
		0.040 0		44 cC		32 cC		12		8		0.000^**		0.000^**			0.040 0		28 bB		20 bB			28	20 bB		1.000								1.000
		0.400 0		64 dG		48 dD		8		4		0.000^**		0.000^**			0.400 0		40 cD		28 cC			36	28 cC		0.646								1.000
		2.000 0		76 eG		56 eE		4		8		0.000^**		0.000^**			2.000 0		32 dC		40 dD			28	40 dD		0.606								0.646
己二酸二异丁酯		0.000 4		16 aB		12 aA		16		12		1.000		1.000	月桂酸		0.000 4		12 aA		12 aA			12	12 aA		1.000								1.000
		0.004 0		32 cC		16 aA		28		16		0.606		1.000			0.004 0		16 bA		20 bA			16	20 bA		1.000								1.000
		0.040 0		28 bB		20 bB		24		20		0.579		1.000			0.040 0		20 bA		32 cC			16	32 cC		0.505								0.606
		0.400 0		36 cD		32 cC		16		28		0.006^**		0.606			0.400 0		32 cC		32 cC			20	32 cC		0.096								0.606
		2.000 0		44 dD		28 cC		16		24		0.000^**		0.579			2.000 0		40 dD		36 cC			16	36 cC		0.001^**								0.317
挥发性化合物 compound	浓度/ (mol·L^-1) concentration		引诱率/% lure rate				驱避率/% repellent rate				P				挥发性化合物 compound	浓度/ (mol·L^-1) concentration										引诱率/% lure rate						驱避率/% repellent rate				P
挥发性化合物 compound	浓度/ (mol·L^-1) concentration		雌 female		雄 male		雌 female		雄 male		雌 female		雄 male		挥发性化合物 compound	浓度/ (mol·L^-1) concentration										雌 female		雄 male		雌 female		雄 male		雌 female		雄 male
邻苯二甲酸二异丁酯	0.000 4		12 aA		12 aA		12		12		1.000		1.000		罗勒烯	0.000 4		12 bA		12 bA		16	12 bA			0.450								0.450
	0.004 0		16 bA		32 cC		16		28		1.000		0.606			0.004 0		16 cA		16 cA		36	16 cA			0.006^**								0.021^*
	0.040 0		32 cB		28 cC		28		24		0.606		0.579			0.040 0		16 c		8b A		48	8b A			0.000^**								0.000^**
	0.400 0		32 cC		28 cC		28		24		0.606		0.579			0.400 0		12 bA		4 aA		64	4 aA			0.000^**								0.000^**
	2.000 0		28 cC		40 dD		24		28		0.579		0.146			2.000 0		4 aA		0 aA		76	0aA			0.000^**								0.000^**
棕榈酸甲酯	0.000 4		12 aA		16 aB		12		16		1.000		1.000		角鲨烯	0.000 4		16 bB		12 bA		16	12 bA			1.000								1.000
	0.004 0		12 aA		36 cC		12		32		1.000		0.628			0.004 0		28 cB		24 cB		32	24 cB			0.606								0.579
	0.040 0		20 bA		28 bC		20		24		1.000		0.579			0.040 0		28 cB		28 cC		40	28 cC			0.146								0.146
	0.400 0		36 cD		40 cD		32		28		0.628		0.146			0.400 0		16 bA		8 aA		44	8 aA			0.000^**								0.000^**
	2.000 0		32 cC		44 dD		28		36		0.606		0.371			2.000 0		4 aA		4 aA		48	4 aA			0.000^**								0.000^**
乙酸叶醇酯	0.000 4		20 aB		20 aB		8		12		0.023^*		0.157		顺-3- 己烯醇	0.000 4		36 bD		32 aC		32	32 aC			0.628								0.606
	0.004 0		48 dE		36 cC		4		12		0.000^**		0.001^**			0.004 0		28 aB		32 aC		24	32 aC			0.579								0.285
	0.040 0		64 eF		52 dE		4		8		0.000^**		0.000^**			0.040 0		48 cD		36 aD		12	36 aD			0.000^**								0.001^**
	0.400 0		72 fH		60 eE		4		4		0.000^**		0.000^**			0.400 0		56 dF		48 cD		12	48 cD			0.000^**								0.000^**
	2.000 0		80 hH		68 eF		0		0		0.000^**		0.000^**			2.000 0		60 eF		52 dE		4	52 dE			0.000^**								0.000^**
丙酸叶醇酯	0.000 4		24 aC		24 aC		12		20		0.046^*		0.054 6		2-己基- 1-癸醇	0.000 4		20 aB		16 aB		20	16 aB			1.000								1.000
	0.004 0		36 cD		36 bC		12		8		0.001^**		0.000^**			0.004 0		32 bC		32 cC		28	32 cC			0.606								0.606
	0.040 0		56 dE		48 cE		12		8		0.000^**		0.000^**			0.040 0		40 cC		28 bC		28	28 bC			0.146								0.579
	0.400 0		64 eG		56 dE		8		4		0.000^**		0.000^**			0.400 0		44 cE		40 dD		20	40dD			0.003^**								0.001^**
	2.000 0		72 fG		64 eF		4		4		0.000^**		0.000^**			2.000 0		52 dE		44 dD		16	44 dD			0.000^**								0.000^**

Table 3

References 38

[1]	萧刚柔. 中国森林昆虫[M]. 北京: 中国林业出版社, 1992.
	XIAO G R. Forest insects of China[M]. Beijing: China Forestry Publishing House, 1992.
[2]	SMITH M T, BANCROFT J, LI G H, et al. Dispersal of Anoplophora glabripennis(Cerambycidae)[J]. Environ Entomol, 2001,30(6):1036-1040.DOI: 10.1603/0046-225x-30.6.1036. doi: 10.1603/0046-225X-30.6.1036
[3]	NOWAK D J, PASEK J E, SEQUEIRA R A, et al. Potential effect of Anoplophora glabripennis (Coleoptera: Cerambycidae) on urban trees in the United States[J]. Journal of Economic Entomology, 2001,94(1):116-122. DOI: 10.1603/0022-0493-94.1.116. doi: 10.1603/0022-0493-94.1.116 pmid: 11233100
[4]	MOREWOOD W D, HOOVER K, NEINER P R, et al. Host tree resistance against the polyphagous wood-boring beetle Anoplophora glabripennis[J]. Entomol Exp Et Appl, 2004,110(1):79-86.DOI: 10.1111/j.0013-8703.2004.00120.x. doi: 10.1111/eea.2004.110.issue-1
[5]	KEENA M A. Anoplophora glabripennis(Coleoptera:Cerambycidae) fecundity and longevity under laboratory conditions:comparison of populations from New York and Illinois on Acer saccharum[J]. Environ Entomol, 2002,31(3):490-498.DOI: 10.1603/0046-225x-31.3.490. doi: 10.1603/0046-225X-31.3.490
[6]	张风娟, 金幼菊, 陈华君, 等. 光肩星天牛对4种不同槭树科寄主植物的选择机制[J]. 生态学报, 2006,26(3):870-877.
	ZHANG F J, JIN Y J, CHEN H J, et al. The selectivity mechanism of Anoplophora glabripennis on four different species of maples[J]. Acta Ecol Sin, 2006,26(3):870-877.DOI: 10.3321/j.issn:1000-0933.2006.03.032.
[7]	张艳菊, 陈霞, 刘海燕, 等. 糖槭叶枯病菌室内药效试验[J]. 东北农业大学学报, 2011,42(1):76-80.
	ZHANG Y J, CHEN X, LIU H Y, et al. Efficacy tests on fungicides of Phyllosticta negundinis in laboratory[J]. J Northeast Agric Univ, 2011,42(1):76-80.DOI: 10.19720/j.cnki.issn.1005-9369.2011.01.014.
[8]	马晓乾, 王茜, 邓勋, 等. 光肩星天牛在糖槭上产卵部位的选择及刻槽产卵习性研究[J]. 安徽农业科学, 2012,40(7):4078-4079, 4107.
	MA X Q, WANG Q, DENG X, et al. Studies on oviposition site selection of Anoplophora glabripennis in Acer saccharum and its gnawing nidus oviposition habits[J]. J Anhui Agric Sci, 2012,40(7):4078-4079, 4107. DOI: 10.13989/j.cnki.0517-6611.2012.07.171.
[9]	贾小俭, 马娟, 高波, 等. 甘薯蚁象对不同甘薯品种植物挥发物的EAG和嗅觉反应[J]. 昆虫学报, 2017,60(11):1285-1291.
	JIA X J, MA J, GAO B, et al. EAG and olfactory responses of Cylas formicarius(Coleoptera:Curculionidae) to volatiles from plants of different sweetpotato cultivars[J]. Acta Entomol Sin, 2017,60(11):1285-1291.DOI: 10.16380/j.kcxb.2017.11.006.
[10]	REYES H, MALO E A, TOLEDO J, et al. Physiological state influences the antennal response of Anastrepha obliqua to male and host volatiles[J]. Physiol Entomol, 2017,42(1):17-25.DOI: 10.1111/phen.12157. doi: 10.1111/phen.2017.42.issue-1
[11]	COLLATZ J, DORN S. A host-plant-derived volatile blend to attract the apple blossom weevil Anthonomus pomorum the essential volatiles include a repellent constituent[J]. Pest Management Science, 2013,69(9):1092-1098. DOI: 10.1002/ps.3477. doi: 10.1002/ps.3477 pmid: 23450733
[12]	ALLISON J D, BORDEN J H, SEYBOLD S J. A review of the chemical ecology of the Cerambycidae (Coleoptera)[J]. Chemoecology, 2004,14(3/4):123-150. DOI: 10.1007/s00049-004-0277-1.
[13]	COOK S M, KHAN Z R, PICKETT J A. The use of push-pull strategies in integrated pest management[J]. Annu Rev Entomol, 2007,52(1):375-400.DOI: 10.1146/annurev.ento.52.110405.091407. doi: 10.1146/annurev.ento.52.110405.091407
[14]	李建光, 骆有庆, 金幼菊. 复叶槭挥发性物质对光肩星天牛的触角电位反应[J]. 北京林业大学学报, 1999,21(4):1-5.
	LI J G, LUO Y Q, JIN Y J. Electroantennogram activity of ash-leaf maple (Acer negundo)volatiles to Anoplophora glabripennis (Motsch.)[J]. J Beijing For Univ, 1999,21(4):1-5.DOI: 10.3321/j.issn:1000-1522.1999.04.001.
[15]	张风娟, 武晓颖, 杨莉, 等. 超临界CO₂萃取五角枫挥发物及其对光肩星天牛的嗅觉行为反应[J]. 林业科学, 2007,43(6):146-150. doi: 10.11707/j.1001-7488.20070626
	ZHANG F J, WU X Y, YANG L, et al. Volatiles on behavior responses of Anoplophora glabripennis by the supercritical carbon dioxide extraction of Acer mono[J]. Sci Silvae Sin, 2007,43(6):146-150.DOI: 10.11707/j.issn:1001-7488.200706026.
[16]	李继泉, 樊慧, 金幼菊, 等. 光肩星天牛取食后复叶槭挥发物的释放机制[J]. 北京林业大学学报, 2002,24(5):170-174.
	LI J Q, FAN H, JIN Y J, et al. Emission mechanism of volatile compounds from ashleaf maple feeding-damaged by Anoplophora glabripennis (Motsch.)[J]. J Beijing For Univ, 2002,24(5):170-174.DOI: 10.3321/j.issn:1000-1522.2002.05.032.
[17]	范丽清, 严善春, 程红, 等. 光肩星天牛对寄主植物萜烯类化合物触角活性和电位反应[J]. 东北林业大学学报, 2012,40(11):143-146.
	FAN L Q, YAN S C, CHENG H, et al. Antennal activity and EAG responses of Asian longhorn beetle Anoplophora glabripennis (Coleoptera:Cerambycidae) to plant terpenes[J]. J Northeast For Univ, 2012,40(11):143-146.DOI: 10.3969/j.issn.1000-5382.2012.11.035.
[18]	范丽清, 严善春, 孙宗华, 等. 光肩星天牛对植物源挥发物的触角电位和行为反应[J]. 生态学杂志, 2013,32(1):142-148.
	FAN L Q, YAN S C, SUN Z H, et al. EAG and behavioral responses of Asian longhorn beetle Anoplophora glabripennis (Coleoptera:Cerambycidae) to plant volatiles[J]. Chin J Ecol, 2013,32(1):142-148.
[19]	李硕, 高薇, 程相称, 等. 光肩星天牛对复叶槭挥发物的触角电位及行为反应[J]. 中国森林病虫, 2016,35(2):9-14.
	LI S, GAO W, CHENG X C, et al. Electroantennogram response of Anoplophora glabripennis (Motsch.) to Acer negundo volatiles[J]. For Pest Dis, 2016,35(2):9-14.DOI: 10.3969/j.issn.1671-0886.2016.02.003.
[20]	ALLISON J D, CARDÉ R T. Male pheromone blend preference function measured in choice and no-choice wind tunnel trials with almond moths,Cadra cautella[J]. Animal Behav, 2008,75(1):259-266.DOI: 10.1016/j.anbehav.2007.04.033. doi: 10.1016/j.anbehav.2007.04.033
[21]	WILLIAMS L III, RODRIGUEZ-SAONA C, CASTLE S C, et al. EAG-active herbivore-induced plant volatiles modify behavioral responses and host attack by an egg parasitoid[J]. J Chem Ecol, 2008,34(9):1190-1201.DOI: 10.1007/s10886-008-9520-5. doi: 10.1007/s10886-008-9520-5
[22]	NGUMBI E, CHEN L, FADAMIRO H Y. Comparative GC-EAD responses of a specialist (Microplitis croceipes) and a generalist (Cotesia marginiventris) parasitoid to cotton volatiles induced by two caterpillar species[J]. J Chem Ecol, 2009,35(9):1009-1020.DOI: 10.1007/s10886-009-9700-y. doi: 10.1007/s10886-009-9700-y
[23]	马艳, 史黎央, 赵艺, 等. 不同危害状态下寄主山核桃挥发物成分的比较及桑天牛对其组分的GC-EAD和行为反应[J]. 昆虫学报, 2018,61(5):574-584.
	MA Y, SHI L Y, ZHAO Y, et al. Comparison of volatiles released from the host Juglans mandshurica in different damaged states and the GC-EAD and behavioral responses of Apriona germari (Coleoptera:Cerambycidae) to these volatiles[J]. Acta Entomol Sin, 2018,61(5):574-584.DOI: 10.16380/j.kcxb.2018.05.007.
[24]	彭璐, 盛平友, 姜瑞凤, 等. 松梢象危害前后红松挥发性物质的变化[J]. 东北林业大学学报, 2009,37(7):99-101.
	PENG L, SHENG P Y, JIANG R F, et al. Volatile changes of Pinus koraiensis before and after damaged by Pissodes nitidus[J]. J Northeast For Univ, 2009,37(7):99-101.DOI: 10.3969/j.issn.1000-5382.2009.07.033.
[25]	王琪, 严善春, 严俊鑫, 等. 健康和虫害的红松挥发物对赤松梢斑螟及其寄生蜂寄主选择行为的影响[J]. 生态学报, 2013,33(23):7437-7444. doi: 10.5846/stxb201208201173
	WANG Q, YAN S C, YAN J X, et al. Effect of volatiles from healthy or worm bored Korean pine on host selective behavior of Dioryctria sylvestrella and its parasitoid Macrocentrus sp.[J]. Acta Ecol Sin, 2013,33(23):7437-7444.DOI: 10.5846/stxb201208201173.
[26]	宁眺, 樊建庭, 方宇凌, 等. 不同危害状态下寄主萜烯挥发物含量的变化及松墨天牛对其组分的触角电位反应[J]. 昆虫学报, 2006,49(2):179-188.
	NING T, FAN J T, FANG Y L, et al. Changes in contents of host volatile terpenes under different damaged states and electroantennogram response of Monochamus alternatus Hope to these volatiles[J]. Acta Entomol Sin, 2006,49(2):179-188.DOI: 10.3321/j.issn:0454-6296.2006.02.003.
[27]	SMID H, VAN LOON J J A, POSTHUMUS M A, et al. GC-EAG-analysis of volatiles from Brussels sprouts plants damaged by two species of Pieris caterpillars:olfactory receptive range of a specialist and a generalist parasitoid wasp species[J]. Chemoecology, 2002,12(4):169-176.DOI: 10.1007/PL00012665. doi: 10.1007/PL00012665
[28]	王保新, 杨桦, 杨伟, 等. 云斑天牛对10种植物挥发物的EAG和行为反应[J]. 应用昆虫学报, 2014,51(2):481-489.
	WANG B X, YANG H, YANG W, et al. EAG and behavioral responses of Batocera lineolata Chevrolat(Coleoptera:Cerambycidae) to ten plant volatiles[J]. Chin J Appl Entomol, 2014,51(2):481-489.
[29]	HERN A, DORN S. A female-specific attractant for the codling moth,Cydia pomonella,from apple fruit volatiles[J]. Naturwissenschaften, 2004,91(2):77-80.DOI: 10.1007/s00114-003-0484-6. doi: 10.1007/s00114-003-0484-6 pmid: 14991144
[30]	TOOKER J F, CRUMRIN A L, HANKS L M. Plant volatiles are behavioral cues for adult females of the gall wasp Antistrophus rufus[J]. Chemoecology, 2005,15(2):85-88.DOI: 10.1007/s00049-005-0298-4. doi: 10.1007/s00049-005-0298-4
[31]	RONALD D Y. SPSS其实很简单[M]. 北京: 中国人民大学出版社, 2010.
	RONALD D Y. SPSS demystified[M]. Beijing: China Renmin University Press, 2010.
[32]	VISSER J H. Host odor perception in phytophagous insects[J]. Annu Rev Entomol, 1986,31(1):121-144.DOI: 10.1146/annurev.en.31.010186.001005. doi: 10.1146/annurev.en.31.010186.001005
[33]	杜永均, 严福顺. 植物挥发性次生物质在植食性昆虫、寄生植物和昆虫天敌关系中的作用机理[J]. 昆虫学报, 1994,37(2):233-250.
	DU Y J, YAN F S. The role of plant volatiles in tritrophicinteractions among phytophagousinsects,their host plants andnatural enemies[J]. Acta Entomol Sin, 1994,37(2):233-250.DOI: 10.16380/j.kcxb.1994.02.020.
[34]	卢伟, 侯茂林, 文吉辉, 等. 植物挥发性次生物质对植食性昆虫的影响[J]. 植物保护, 2007,33(3):7-11.
	LU W, HOU M L, WEN J H, et al. Effects of plant volatiles on herbivorous insects[J]. Plant Prot, 2007,33(3):7-11.DOI: 10.3969/j.issn.0529-1542.2007.03.002.
[35]	王紫薇, 徐华潮, 张娓娓, 等. 光肩星天牛对寄主的选择及主要寄主挥发物的化学成分分析[J]. 浙江农林大学学报, 2016,33(4):558-563.
	WANG Z W, XU H C, ZHANG W W, et al. Anoplophora glabripennis host-plant selection with main host-plant volatile chemical component analysis[J]. J Zhejiang A & F Univ, 2016,33(4):558-563.DOI: 10.11833/j.issn.2095-0756.2016.04.002.
[36]	刘曼, 任春光, 杨茂发, 等. 竹织叶野螟触角感器的超微形态特征[J]. 林业科学, 2013,49(9):107-111.
	LIU M, REN C G, YANG M F, et al. Ultrastructures of the antennal sensilla in algedomia coclesalis[J]. Sci Silvae Sin, 2013,49(9):107-111.DOI: 10.11707/j.1001-7488.20130915.
[37]	WEE S L, OH H W, PARK K C. Antennal sensillum morphology and electrophysiological responses of olfactory receptor neurons in trichoid sensilla of the diamondback moth (Lepidoptera:Plutellidae)[J]. Flo Entomol, 2016,99(S1):146-158.DOI: 10.1653/024.099.sp118.
[38]	阎雄飞, 孙月琴, 刘永华, 等. 光肩星天牛触角感受器的环境扫描电镜观察[J]. 林业科学, 2010, 46(11):104-109, 191-194. doi: 10.11707/j.1001-7488.20101032
	YAN X F, SUN Y Q, LIU Y H, et al. Environmental scanning electron microscopic observations of sensilla on the antennae of Anoplophora glabripennis[J]. Sci Silvae Sin, 2010, 46(11): 104-109, 191-194. DOI: 10.11707/j.1001-7488.20101115.

Metrics

Comments

www.nldxb.njfu.edu.cn

Edited ＆ Published by Editorial Department of Nanjing Forestry University(Natural Sciences Edition)
Address： No.159 Longpan Road,Nanjing 210037 Jiangsu,P.R.China
E-mail ：xuebao@njfu.edu.cn , xuebao@njfu.com.cn
Telephone +86-25-85428247,85427076
Distributed by China International Book Trading Corporation P.O. Box 399, Beijing ,P.R. China

序号 No.	化合物名称 compound	分子式 formula	相对含量/% relative content	保留时间/s retention time	序号 No.	化合物名称 compound	分子式 formula	相对含量/% relative content	保留时间/s retention time
1	3-戊醇	C₅H₁₂O	0.25	2.222	18	十四酸	C₁₄H₂₈O₂	1.24	21.660
2	顺-3-己烯醇	C₆H₁₂O	3.22	5.555	19	十九烷	C₁₉H₄₀	0.25	22.230
3	乙酸叶醇酯	C₈H₁₄O₂	33.06	10.445	20	邻苯二甲酸二异丁酯	C₁₆H₂₂O₄	3.51	22.450
4	乙酸己酯	C₈H₁₆O₂	1.99	10.700	21	R-(Z)-9-辛烯酸-12- (乙酰基)-甲基酯	C₂₁H₃₈O₄	1.02	22.890
5	罗勒烯	C₁₀H₁₆	2.12	11.711	22	2-羟基-环十五烷酮	C₁₅H₂₈O₂	0.65	23.090
6	丙酸叶醇酯	C₉H₁₆O₂	1.16	12.480	23	酞酸二丁酯	C₁₆H₂₂O₄	0.38	24.390
7	棕榈酸甲酯	C₁₀H₁₈O₂	10.08	13.240	24	十五烷酸	C₁₅H₃₀O₂	0.48	24.540
8	丁酸己酯	C₁₀H₂₀O₂	0.27	13.530	25	二十一烷	C₂₁H₄₄	0.25	25.430
9	戊酸-3-己烯酯	C₁₁H₂₀O₂	0.49	14.160	26	十四环氧乙烷	C₁₆H₃₂O	0.42	26.320
10	异戊酸-顺-3-己烯酯	C₁₁H₂₀O₂	0.50	14.760	27	十五醇	C₁₅H₃₂O	0.46	28.060
11	4-己烯-己酸酯	C₁₂H₂₂O₂	0.21	15.190	28	2-己基-1-癸醇	C₁₆H₃₄O	1.09	29.540
12	月桂酸	C₁₂H₂₄O₂	3.21	16.580	29	硬脂酸甲酯	C₁₉H₃₈O₂	1.12	31.690
13	顺-3-己烯-苯甲酸酯	C₁₃H₁₆O₂	0.48	17.870	30	(Z)-13-十八碳烯醛	C₁₈H₃₄O	0.60	32.970
14	甲基,双(1-甲基丙基)- 丁二酸酯	C₁₃H₂₄O₄	0.87	18.350	31	十八烷酸	C₁₈H₃₆O₂	0.65	33.470
15	正十四烷	C₁₄H₃₀	0.21	19.620	32	二十二烷	C₂₂H₄₆	0.41	35.530
16	癸酸环己基酯	C₁₆H₃₀O₂	0.34	20.320	33	邻苯二甲酸二辛酯	C₂₄H₃₈O₄	0.79	37.270
17	己二酸二异丁酯	C₁₄H₂₆O₄	5.40	20.570	34	角鲨烯	C₃₀H₅₀	10.87	39.590

EAG and olfactory behavioral responses of Anoplophora glabripennis (Coleoptera: Cerambycidae) to volatiles of Acer saccharum

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 3

References 38

Related Articles 7

Recommended Articles

Metrics

Comments

Author

Reader

Reviewer

[1]	SUN Long, PENG Zuodeng,WANG Chong,YANG Teng. Effect of drought stress on photosynthetic characteristics of two energy resource sandy shrubs [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2014, 57(02): 99-104.
[2]	MA Xiaoqian,ZHAO Hongying,ZHOU Qi,DENG Xun,SONG Xiaoshuang,YU Wenjing,FAN Haijuan. Composition analysis of the oviposition secretion of Anoplophora glabripennis [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2013, 56(01): 83-86.
[3]	CHEN Yong, LIN Zhongxiang. Oxidation on ring B of dehydroabietylamine derivatives using CrO₃/SiO₂ solid-supported oxidizing reagent [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2012, 55(03): 101-104.
[4]	ZHAO Han1, ZHANG Huaxin1*, LI Fengming2,LIU Zhengxiang1. Type division of Elaeagnus mollis fruits in natural populations in Shanxi province [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2011, 54(03): 11-.
[5]	SHI Yu-ping, WANG Yong-ning, GUO Zhen. Extraction of the Flavonoids from Elaeagnus angnstifolia L. [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2005, 48(02): 106-108.
[6]	ZHANG Yun ling 1,CHANG De long 1,HUANG Wen hao 1,LI Fu hai 1,ZHU Xiu ping 2. Effects of Dilute Alkali Solution and Anti stain Reagents Treatments to the Color of Paulownia Timber [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2001, 44(01): 49-52.
[7]	Feng Huiming Zhang Guowei Shan Qun. THE MANUFACTURING METHOD OF REAGENT TANNIC ACID [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 1990, 33(04): 84-89.