Sequence characteristics and expression pattern analyses of PuZFP103 gene under abiotic stress in Populus ussuriensis

doi:10.12302/j.issn.1000-2006.201911038

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2021, Vol. 45 ›› Issue (1): 36-44.doi: 10.12302/j.issn.1000-2006.201911038

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Sequence characteristics and expression pattern analyses of PuZFP103 gene under abiotic stress in Populus ussuriensis

ZHANG Xin(), MA Miaomiao, LYU Wanqiu, LEE Joobin, YANG Jingli^*()

State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China

Received:2019-11-18 Accepted:2020-03-02 Online:2021-01-30 Published:2021-02-01
Contact: YANG Jingli E-mail:13199385849@163.com;yifan85831647@163.com

Abstract

Abstract:

【Objective】 This study aimed to investigate the function of the PuZFP103 gene in the growth and development process of Populus ussuriensis Kom. We analyzed the sequence characteristics, expression patterns in different tissues, and the PuZFP103 gene response to hormones and abiotic stresses. 【Method】 The sequence characteristics of the PuZFP103 gene were analyzed using bioinformatics methods. The expression characteristics of the PuZFP103 gene in different tissues, as well as its response to hormones and abiotic stresses, were analyzed by real-time quantitative PCR (RT-qPCR). In addition, the subcellular localization of the PuZFP103 gene was detected using particle bombardment. 【Result】 The bioinformatic analysis showed that the PuZFP103 gene had 505 bp coding sequences, which encoded a polypeptide of 168 amino acids. The conserved domain analysis revealed that there was a conserved ZnF-C2H2 domain in the PuZFP103 protein. The secondary structure analysis of PuZFP103 showed that the percentage of amino acids of α-helix, random coil, and β-sheet were 24%, 84% and 1%, respectively. The evolutionary tree map showed that it was closely related to the common P. ussuriensis, P. euphratica, P. tomentosa, and Carica papaya. The results of RT-qPCR analysis demonstrated that the expression level of PuZFP103 was increased after NaCl, ABA and PEG 6000 treatment. The subcellular localization assay showed that PuZFP103 was a nuclear protein. 【Conclusion】 The results indicate that the gene is positively regulated by NaCl, ABA and PEG 6000. The PuZFP103 gene may be involved in the metabolic and signaling pathways of osmotic and salt stress and, thus, is a regulatory factor under stress. This study provides preliminarily evidence that the PuZFP103 gene is associated with stress response, but further studies on its signal transduction and regulation mechanism are needed. This is the first study to perform bioinformatics analysis of the PuZFP103 gene in P. ussuriensis.

Key words: Populus ussuriensis, PuZFP103 gene, abiotic stress, bioinformation, RT-qPCR

CLC Number:

S722.5

ZHANG Xin, MA Miaomiao, LYU Wanqiu, LEE Joobin, YANG Jingli. Sequence characteristics and expression pattern analyses of PuZFP103 gene under abiotic stress in Populus ussuriensis[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2021, 45(1): 36-44.

Figures/Tables 9

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Sequence characteristics and expression pattern analyses of PuZFP103 gene under abiotic stress in Populus ussuriensis

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