Effects of mechanical damage treatment on the tillering ability and endogenous hormone content of Chinese fir clones

doi:10.12302/j.issn.1000-2006.202006054

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2021, Vol. 45 ›› Issue (2): 153-158.doi: 10.12302/j.issn.1000-2006.202006054

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Effects of mechanical damage treatment on the tillering ability and endogenous hormone content of Chinese fir clones

ZHANG Jiling¹(), LI Mingyang², LI Yong³, LIU Li¹, FEI Yuchong¹, CAO Guangqiu¹^,⁴^,^*()

1. College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China
2. College of Plant Protection, Fujian Agricultural and Forestry University, Fuzhou 350002, China
3. Fujian Yangkou State-owned Forest Farm, Shunchang 353200, China
4. China Fir Engineering Research Center, National Forestry and Grassland Administration, Fuzhou 350002, China

Received:2020-06-29 Accepted:2020-11-09 Online:2021-03-30 Published:2021-04-09
Contact: CAO Guangqiu E-mail:2325953750@qq.com;cncgq@126.com

Abstract

Abstract:

【Objective】The effect of mechanical damage on the sprouting ability of Cunninghamia lanceolata (Chinese fir) clones with different propagation methods was analyzed, and the sprouting mechanism was expounded from the perspective of endogenous hormone metabolism.【Method】Based on the analysis of the effects of base damage and topping treatments on the sprouting ability of Chinese fir clone 020 cuttings and 020 tissue culture seedlings, indoleacetic acid (IAA), gibberellin (GA₃), abscisic acid (ABA) and cytckinin (CTK) in branches and leaves, base phloem, root tip and other organs of Chinese fir clone at the peak of sprouting were further determined by enzyme-linked immunosorbent assay (ELISA). Pearson method was used to analyze the correlation between sprouting ability and endogenous hormone content.【Result】The number of sprouting tillers increased with the extension of culture time. The peak of sprouting tillers of 020 cuttings and 020 tissue culture seedlings in topping and base damage treatments were in July. The sprouting ability of topping treatments was significantly higher than that of base damage treatments, and the difference reached a significant level (P < 0.05); with the growth of seedlings, IAA content in branches and leaves of Chinese fir clones increased, GA₃ content decreased; topping treatment was conducive to ABA accumulation in branches and leaves and root tips of Chinese fir seedlings, and basal damage treatment was conducive to CTK accumulation in phloem and root tips.【Conclusion】Under the condition of mechanical injury, IAA, ABA and GA₃ endogenous hormone contents in root tips, branches and leaves of Chinese fir were higher. There was a significant positive correlation between the number of sprouting tillers and the mechanical injury treatments, and a significant positive correlation between the number of sprouting tillers and the contents of GA₃, and a significant negative correlation between the number of sprouting tillers and the content of IAA and ABA.

Key words: Cunninghamia lanceolata (Chinese fir) clone, mechanical damage, tillering, endogenous hormones

CLC Number:

S718

ZHANG Jiling, LI Mingyang, LI Yong, LIU Li, FEI Yuchong, CAO Guangqiu. Effects of mechanical damage treatment on the tillering ability and endogenous hormone content of Chinese fir clones[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2021, 45(2): 153-158.

Figures/Tables 5

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Table 1

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References 29

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无性系 clone	处理 treatment	新增萌芽数number of new sprouts
无性系 clone	处理 treatment	2018-04	2018-05	2018-06	2018-07	2018-08
扦插苗 cutting seedlings	基部损伤 basal injury			1.20±0.20 ab	2.10±0.41 b	1.02±0.21 bc
扦插苗 cutting seedlings	去顶topping			1.54±0.22 b	3.12±0.61 a	1.32±0.23 bc
组培苗 tissue culture seedling	基部损伤 basal injury			1.41±0.21 ab	2.32±0.42 bc	1.25±0.23 a
组培苗 tissue culture seedling	去顶topping			1.60±0.23 ab	3.21±0.45 ac	1.41±0.21 bc

项目 item	繁殖方式 mode of reproduction	处理 treatment	器官部位 organ location	萌蘖数 number of sprouts	吲哚乙酸 IAA	赤霉素 GA₃	脱落酸 ABA	细胞分裂素 CTK
繁殖方式mode of reproduction	1.000
处理treatment	0.000	1.000
器官部位organ location	0.000	0.000	1.000
萌蘖数number of sprouts	0.000	0.001^*	0.000	1.000
吲哚乙酸IAA	0.154	-0.286^*	-0.292^*	-0.001^*	1.000
赤霉素GA₃	0.093	0.201	0.064	0.096^*	-0.354^**	1.000
脱落酸ABA	0.234^*	0.222	0.067	-0.193^*	-0.084	0.215	1.000
细胞分裂素CTK	0.038	-0.071	-0.136	-0.210	-0.106	0.010	0.106	1.000

Effects of mechanical damage treatment on the tillering ability and endogenous hormone content of Chinese fir clones

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