JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2021, Vol. 45 ›› Issue (5): 10-18.doi: 10.12302/j.issn.1000-2006.202012046
Special Issue: 专题报道
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YUAN Jinling1, MA Jingxia2, ZHONG Yuanbiao1, YUE Jinjun1
Received:2020-12-28
Accepted:2021-03-10
Online:2021-09-30
Published:2021-09-30
CLC Number:
YUAN Jinling, MA Jingxia, ZHONG Yuanbiao, YUE Jinjun. SSR-based hybrid identification, genetic analyses and fingerprint development of hybridization progenies from sympodial bamboo (Bambusoideae, Poaceae)[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2021, 45(5): 10-18.
Table 1
Thirty SSR primers randomly selected and their sequence"
| No. 序号 | marker 引物 名称 | primer sequence (5'→ 3') 引物序列 | No. 序号 | marker 引物 名称 | primer sequence (5'→ 3') 引物序列 | |||
|---|---|---|---|---|---|---|---|---|
| F-primer正向 | R-primer反向 | F-primer 正向 | R-primer 反向 | |||||
| P1 | PBM031 | CGCCGAGTTCCCTATTATTATTT | AGCACAGCCTCCGTGATTG | P16 | DOM01 | TGGAGGCATTAAGCACAG | TTGACACTCGTGGTAGGC | |
| P2 | PBM032 | TTTCCCAAATAAAACCTCACC | GTCCATTTAGGGTTCCACTGA | P17 | DOM02 | GTTCAGCCCCTTACCTCC | TGATCAATTCAGATTCCACTT | |
| P3 | PBM033 | CTGACTGTGCGTGCGTCTC | CTTGGTCTCGCTCATCTCCTC | P18 | DOM03 | AAGGAGTTCATCTCCCGACA | CACCACGCAAACCAAGCT | |
| P4 | PBM034 | TCGGCTCGGCGTGATGGAT | ATCGGCATCCGCGACTGCC | P19 | DOM04 | GGAGTTCATCTCCCGACA | TCTTTAAGGCAAACAACG | |
| P5 | PBM035 | ACCGTGATGACTACCGCCGCGACC | TGCTGCCTCCACCCCTCCGTCC | P20 | DOM05 | TTGGAGGAGACTGAATGA | CCTTTGCGAAAATACACG | |
| P6 | PBM036 | CACATGGACCGCCTCATCC | GCAACAAAACGAGAACCAGAC | P21 | DOM06 | TCTAGCATGGACGGTCAG | TCTCGCATAAGCCACATC | |
| P7 | PBM037 | TGCAAGCCTGCTATACGTTT | GAAGTGGGAGTACATACTTCCCA | P22 | DOM07 | GGGCTGTCTGGCTTGTTA | TGAAAGGATAGGTTAGGTTGC | |
| P8 | PBM038 | GGTCGGCTCATTTTGTAGTGT | CAACCTTCAGGCAATAGATTACAT | P23 | DOM08 | CCTGAAGGTTGACTGGGATA | TCCTCCGCCTTAGTGTCT | |
| P9 | PBM039 | CATCCTCAGTTTCTCACCG | CAGCTTCACCAACTTGTGG | P24 | DOM09 | CACCACGCAAACCAAGCT | GGAGTTCATCTCCCGACA | |
| P10 | PBM040 | GAATCATCTGGGAAGAAGAAGGA | TGCATTGCATTTGGCTTAGTAGT | P25 | DOM10 | TGCTTCATTATCTGCTGGTT | TGCACGTCGCAAGTTTCA | |
| P11 | PBM041 | TGGTGTTGCCTGTGACCTTAC | CCCACCTCCACCTCTACTACG | P26 | DOM11 | GCCCGACTTTCTTTCATCTCCC | CGCATTTCGCTACGTTCTTCAT | |
| P12 | PBM042 | TCCTTTACGGCTTTACCCC | GCCCCAGCTTAGTACACCAC | P27 | DOM12 | CCGGAGCGATCTCAAGGA | CCAAGAGTCTGGGAGTAGGG | |
| P13 | PBM043 | CTCACCGCCCCACCTCGCA | CGGCTGCTGATGCGGAGGA | P28 | DOM13 | CCTGACCGAAGCAGACCG | GCCGAGATATCCGTTGCC | |
| P14 | PBM044 | AAGGCCCACGTTGCCAGAC | GTTCCCGTTGATGCCCCAC | P29 | DOM14 | GTGCCTTCCCAGTTTGTC | CCTTTGCTATTGCCTTGC | |
| P15 | PBM045 | TGAGCGAGGTAGTTTCATTTTAGTTA | CCTACGACGAGTAGATTGCGAGT | P30 | DOM15 | ATCCTAGTGCGCCTCGTC | TTGGTGGGCTCCCTTTGT | |
Fig.1
Electropherogram profiles of E1-E10 and A1-A9 under PCR amplification with primer pairs P8, P9 and P19"
Fig.2
Electropherogram profiles of E, A and B1-B34 under the PCR amplification of primer P8 and P27"
Fig.3
Dendrogram of A, E and their 34 E×A hybrids under cluster analysis using alleles detected from six SSR primers (P8, P9, P18, P19, P20 and P27)"
表2
Fingerprints of 34 hybrids from E × A cross using alleles detected from SSR primers of P8, P20 and P27"
| primer 引物 | B1 | B2 | B3 | B4 | B5 | B6 | B7 | B8 | B9 | B10 | B11 | B12 | B13 | B14 | B15 | B16 | B17 | B18 | B19 | B20 | B21 | B22 | B23 | B24 | B25 | B26 | B27 | B28 | B29 | B30 | B31 | B32 | B33 | B34 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 1 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | |
| P8 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |
| 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | |
| 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | |
| 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | |
| 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | |
| P20 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 |
| 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | |
| 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | |
| 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | |
| 1 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | |
| P27 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
| 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
Fig.4
Electropherogram profiles of E, C and D1-D59 under the PCR amplification of primer P9"
Fig.5
Dendrogram of E, C and their 59 suspected hybrids under cluster analysis using alleles detected with six SSR primers(P2, P9, P10, P11, P20 and P28)"
表3
Fingerprints of 54 true hybrids from E × C cross using alleles detected with SSR primers of P2, P9 and P11"
| primer 引物 | D1 | D2 | D3 | D4 | D5 | D6 | D7 | D8 | D9 | D10 | D11 | D12 | D13 | D14 | D15 | D16 | D17 | D18 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| P2 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | |
| 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |
| P9 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | |
| 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |
| P11 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 |
| 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | |
| 0 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 0 | 1 | |
| 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | |
| 1 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | |
| primer 引物 | D19 | D20 | D22 | D23 | D24 | D25 | D26 | D27 | D28 | D29 | D30 | D31 | D32 | D33 | D34 | D35 | D36 | D37 |
| P2 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 0 | 1 | |
| 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |
| P9 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | |
| 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |
| P11 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
| 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | |
| 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
| 1 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | |
| primer 引物 | D38 | D39 | D40 | D41 | D42 | D43 | D44 | D46 | D47 | D49 | D50 | D51 | D52 | D53 | D54 | D55 | D56 | D59 |
| P2 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | |
| 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |
| P9 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| 0 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | |
| 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |
| P11 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | |
| 1 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | |
| 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
| 0 | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 1 |
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