Dynamic changes in nutrients content and related enzymes activity during Paeonia ostii ‘Feng Dan’ seeds development

doi:10.12302/j.issn.1000-2006.202012039

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2021, Vol. 45 ›› Issue (5): 62-70.doi: 10.12302/j.issn.1000-2006.202012039

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Dynamic changes in nutrients content and related enzymes activity during Paeonia ostii ‘Feng Dan’ seeds development

ZOU Yuting¹(), ZHU Mingwei¹, LI Yongrong², ZHAI Jinting³, LI Shuxian¹^,^*()

Received:2020-12-27 Accepted:2021-03-11 Online:2021-09-30 Published:2021-09-30
Contact: LI Shuxian E-mail:854504350@qq.com;shuxianli@njfu.com.cn

Abstract

Abstract:

【Objective】 To understand the developmental characteristics of Paeonia ostii ‘Feng Dan’ seed and to provide theoretical basis for their scientific cultivation and management, we studied the morphological changes during P. ostii ‘Feng Dan’ follicle as well as seed development and discussed the transformation of nutrients during P. ostii ‘Feng Dan’ seed development. 【Method】 The P. ostii ‘Feng Dan’ follicles and seeds were stratified at six stages and used as materials for biological observation. Additionally, indexes such as water, soluble sugar, soluble protein, crude starch, crude lipid content, total amylase, total protease, acetyl-CoA carboxylase (ACCase), and phosphatidate phosphatase (PPase) activities in seeds were studied. Changes in nutrients and their related enzyme activities during seed development were analyzed. 【Result】 P. ostii ‘Feng Dan’ seed development was divided into two stages: green ripe stage (45-85 days after flowering) and brown ripe stage (95-115 days after flowering). During this process, the water content of P. ostii ‘Feng Dan’ seed decreased significantly, and the seed coat changed from yellow to white. Finally, the coat was hard and black brown. The pericarp also changed from green to yellowish brown and finally showed as grayish brown with dehiscent pods. Results of nutrient and related enzyme activity determination showed that during P. ostii ‘Feng Dan’ seed development, the oil accumulation flourished mainly at 45-85 days after flowering, increasing slowly at the middle and later stages, and reached the highest value of 33.1% at maturity. In addition to the consumption trend of soluble sugar corresponding to the accumulation trend of soluble protein, the change in soluble sugar content also showed a significant positive correlation with the change in crude starch content, showing downward-rising decreasing trends, and all reached the lowest value at 85 days after flowering. During the process, the trend of total amylase activity was opposite to that of crude starch, and the trends of total protease and soluble protein were the same. When the content of crude starch decreased with an increase in total amylase activity, the content of soluble protein increased with a decrease in total protease activity. ACCase and PPase activities also increased with an increase in lipid accumulation rate. 【Conclusion】 The changing trend of nutrient contents and related biological characteristics indicated that it is more appropriate to harvest P. ostii ‘Feng Dan’ seed at approximately 115 days after flowering. Additionally, distinct management schemes must be adopted at different stages of the planting process. A sufficient supply of water and phosphorus fertilizer should be ensured at 45-65 days after flowering. At 65-85 days after flowering, shallow irrigation should be used, and an appropriate amount of nitrogen and potassium fertilizer should be applied. However, in the middle and later stages of seed development, the water supply should be further reduced and a small amount of topdressing phosphorus should be reapplied.

Key words: Paeonia ostia ‘Feng Dan’, seeds development, nutrient, oil, enzymes activity

CLC Number:

S727.3

ZOU Yuting, ZHU Mingwei, LI Yongrong, ZHAI Jinting, LI Shuxian. Dynamic changes in nutrients content and related enzymes activity during Paeonia ostii ‘Feng Dan’ seeds development[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2021, 45(5): 62-70.

Figures/Tables 6

Table 1

Fig.1

Fig.2

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Fig.4

Table 2

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花后时间/d days after flowering	蓇葖果 follicles
花后时间/d days after flowering	横径/mm horizontal diameter	纵径/mm vertical diameter	横径/mm horizontal diameter	纵径/mm vertical diameter
45	15.8±1.8 c	39.2±2.9 c	6.3±0.7 c	8.4±0.6 c
65	16.1±2.0 c	45.4±3.9 ab	7.2±0.9 b	9.6±0.5 b
85	17.8±1.6 b	47.0±3.9 a	8.1±0.6 a	10.7±0.6 a
95	18.2±2.4 ab	45.7±3.7 a	8.4±0.7 a	11.1±0.7 a
105	18.2±1.0 ab	44.3±2.3 b	8.3±0.8 a	10.7±0.1 a
115	18.9±2.3 a	44.3±3.9 b	8.1±1.0 a	9.9±0.5 b

指标 index	可溶性糖 soluble sugar	可溶性蛋白 soluble protein	粗淀粉 crude starch	粗脂肪 crude lipid	乙酰辅酶A 羧化酶 Accase	磷脂酸磷酸酯酶 PPase	总淀粉酶 total amylase	总蛋白酶 total protease
可溶性糖 soluble sugar	1
可溶性蛋白 soluble protein	-0.92^**	1
粗淀粉 crude starch	0.82^*	-0.80^*	1
粗脂肪 crude lipid	-0.77	0.93^**	-0.59	1
乙酰辅酶A羧化酶 acetyl-CoA carboxylase	-0.75	0.64	-0.27	0.69	1
磷脂酸磷酸酯酶 phosphatidate phosphatase	-0.68	0.67	-0.93^**	0.43	0.09	1
总淀粉酶 total amylase	-0.66	0.57	-0.79	0.25	0.09	0.92^**	1
总蛋白酶 total protease	0.86^*	-0.67	0.66	-0.44	-0.62	-0.69	-0.82^*	1

Dynamic changes in nutrients content and related enzymes activity during Paeonia ostii ‘Feng Dan’ seeds development

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