Scanning electron microscopy observation on the antennal sensilla of Ooencyrtus kuvanae

doi:10.12302/j.issn.1000-2006.202106012

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2022, Vol. 46 ›› Issue (3): 157-164.doi: 10.12302/j.issn.1000-2006.202106012

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Scanning electron microscopy observation on the antennal sensilla of Ooencyrtus kuvanae

WANG Jianjun¹^,²(), WANG Jingxian²^,^*(), LUAN Qingshu², DU Hui³, JIANG Xu², DONG Lili², CAO Chuanwang¹

1. College of Forestry, Northeast Forestry University, Harbin 150040, China
2. Liaoning Provincial Key Laboratory of Forest Protection, Liaoning Academy of Forestry Sciences, Shenyang 110032, China
3. Anshan City Natural Resources Services Centre of Liaoning Province, Anshan 114000, China

Received:2021-06-12 Accepted:2021-12-29 Online:2022-05-30 Published:2022-06-10
Contact: WANG Jingxian E-mail:jjw653723@126.com;wangjingxian_1985@163.com

Abstract

Abstract:

【Objective】The type, number and distribution characteristics of the antennal sensilla of male and female Ooencyrtus kuvanae were determined, to provide a basis for exploring the internal structure, sensing mechanisms and related functions of each type of sensilla.【Method】The antenna structure, length and morphological characteristics of antennal sensilla of O. kuvanae were observed using scanning electron microscopy (SEM). The types of sensilla were identified, the number and location were counted for different types of sensilla on the back and ventral surfaces of the antennae. The length of the antenna and the length, width and number of the same type of sensilla between the female and male were compared using an independent sample t test. The differences in the number and distribution density of the sensilla on different antennal segments were compared between the female and male.【Result】The antennae of O. kuvanae were geniculate and the antennae length of the female (806.79 ± 4.30) μm was larger than that of the male (781.13 ± 7.13) μm. The flagellum of the female had 9 segments, including 6 funicles and 3 clavae. The flagellum of the male comprised 7 segments, including 6 funicles and 1 clava. There were 7 types of sensilla on the antennae of both the female and male, including trichomes sensilla (TS), chaetica sensilla (ChS), campullacea sensilla (CaS), placodea sensilla (PS), mammilliformia sensilla (MS), Böhm bristles (BB) and finger-like sensilla (FS). The TS and FS were divided into typeⅠand typeⅡ. The numbers of TS on the female and male were the largest and the most widely distributed. There was no significant difference in the numbers of TSⅠand TSⅡbetween the female and male. The numbers of ChS and FSⅠon the female were significantly greater than that on the male. The numbers of BB and FSⅡon the female were significantly less than that on the male. There was no significant difference in the numbers of PS, CaS and MS between the female and male. The types, number and density of sensilla on the antennae increased from the base to the end.【Conclusion】The total quantity of antennal sensilla on the female and male were similar, but the number, size and distribution of each type of sensilla were different, with pronounced sexual dimorphism. The CaS was found in Encyrtidae. The flagellum of O. kuvanae may play a more important role in the process of host localization, recognition and oviposition.

Key words: Ooencyrtus kuvanae, parasitoid, Lymantria dispar, antennal sensilla, scanning electron microscopy (SEM), sexual dimorphism

CLC Number:

WANG Jianjun, WANG Jingxian, LUAN Qingshu, DU Hui, JIANG Xu, DONG Lili, CAO Chuanwang. Scanning electron microscopy observation on the antennal sensilla of Ooencyrtus kuvanae[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2022, 46(3): 157-164.

Figures/Tables 5

Fig.1

Table 1

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Table 2

Table 3

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性别 gender	支角突 antennifer	柄节 scape	梗节 pedicel	鞭节flagellum										触角全长 overall length of antenna
性别 gender	支角突 antennifer	柄节 scape	梗节 pedicel	F1	F2	F3	F4	F5	F6	C1	C2	C3	F1—C3	触角全长 overall length of antenna
雌蜂female	57.90± 2.75 a	227.20± 4.58 a	80.52± 2.40 a	36.36± 1.16 b	42.50± 0.78 b	48.52± 1.10 b	49.14± 1.64 b	50.52± 0.50 b	44.18± 0.61 b	56.24± 1.63 b	41.48± 0.73	51.46± 2.25	428.33± 3.59 b	806.79± 4.30 a
雄蜂male	45.55± 2.17 b	188.20± 2.58 b	51.60± 2.83 b	51.46± 1.06 a	55.40± 1.22 a	62.58± 1.77 a	63.44± 1.02 a	63.88± 1.16 a	59.12± 1.42 a	129.60± 1.91 a	—	—	492.10± 2.04 a	781.13± 7.13 b

感受器类型 type of sensilla	长度/μm length		基部直径/μm basal diameter		数量 number
感受器类型 type of sensilla	雌蜂female	雄蜂male	雌蜂female	雄蜂male	雌蜂female	雄蜂male
毛形感受器Ⅰ型 TSⅠ	22.95±0.65 b	30.53±0.48 a	1.11±0.03 b	1.41±0.02 a	82.50±13.43 a	104.60±8.28 a
毛形感受器Ⅱ型 TSⅡ	8.90±0.49 b	19.11±0.51 a	0.88±0.04 a	0.89±0.03 a	110.25±24.34 a	102.40±10.41 a
刺形感受器 ChS	20.28±0.95 a	22.68±1.14 a	1.07±0.03 a	1.12±0.04 a	41.75±5.01 a	19.80±1.69 b
钟形感受器CaS	—	—	4.05±0.24 a	3.87±0.11 a	1.80±0.20 a	1.20±0.20 a
板形感受器 PS	30.72±1.00 b	35.19±0.68 a	3.12±0.11 a	3.06±0.17 a	16.00±2.86 a	10.00±2.91 a
Böhm氏鬃毛 BB	3.73±0.16 a	5.37±0.35 b	0.84±0.07 a	0.80±0.05 a	8.00±0.71 b	10.75±0.75 a
乳状感受器 MS	4.14±0.16 a	3.53±0.14 b	1.23±0.07 a	1.31±0.06 a	6.25±3.42 a	4.80±2.40 a
指形感受器Ⅰ型 FSⅠ	7.92±0.29 a	9.79±1.56 a	0.68±0.08 a	0.94±0.13 a	7.67±0.33 a	1.20±0.20 b
指形感受器Ⅱ型 FSⅡ	7.13±0.06 b	11.03±0.26 a	1.36±0.19 a	1.72±0.07 a	4.67±0.33 b	7.20±0.67 a
合计total	—	—	—	—	267.25±36.55 a	250.40±8.21 a

亚节 segments		感受器类型types		感受器数量 number		数量密度/(个·μm^-1) density
亚节 segments		雌蜂female	雄蜂male	雌蜂female	雄蜂male	雌蜂female	雄蜂male
支角突antennifer		BB	BB	7.25±1.03 a	5.80±1.66 a	0.13±0.02 a	0.13±0.04 a
柄节scape		ChS	ChS	29.75±4.39 a	15.60±3.11 b	0.13±0.02 a	0.08±0.02 a
梗节pedicel		BB、ChS、CaS	BB、ChS、CaS、MS	12.75±2.10 a	9.40±1.03 a	0.16±0.03 a	0.18±0.02 a
鞭节 flagellum	F1	TSⅠ、MS	TSⅠ、TSⅡ、MS	10.00±1.47 b	20.80±1.62 a	0.28±0.04 b	0.40±0.03 a
	F2	TSⅠ、TSⅡ、MS、PS	TSⅠ、TSⅡ、MS、PS	12.50±1.04 b	20.00±1.14 a	0.29±0.02 a	0.36±0.02 a
	F3	TSⅠ、TSⅡ、MS、PS	TSⅠ、TSⅡ、MS、PS	18.75±1.65 a	20.00±1.30 a	0.39±0.03 a	0.32±0.02 a
	F4	TSⅠ、TSⅡ、MS、PS	TSⅠ、TSⅡ、MS、PS	17.75±1.11 b	22.60±1.57 a	0.36±0.02 a	0.36±0.02 a
	F5	TSⅠ、TSⅡ、MS、PS	TSⅠ、TSⅡ、MS、PS	20.25±1.60 a	21.60±2.16 a	0.40±0.03 a	0.34±0.03 a
	F6	TSⅠ、TSⅡ、MS、PS	TSⅠ、TSⅡ、MS、PS	19.50±2.40 a	24.40±3.09 a	0.44±0.05 a	0.41±0.05 a
	C1	TSⅠ、TSⅡ、MS、PS	TSⅠ、TSⅡ、MS、 PS、FSⅠ、FSⅡ	36.75±5.50 b	90.20±4.62 a	0.65±0.10 a	0.70±0.04 a
	C2	TSⅠ、TSⅡ、MS、PS	—	34.50±13.84	—	0.83±0.33	—
	C3	TSⅠ、TSⅡ、MS、 PS、FSⅠ、FSⅡ	—	47.50±15.92	—	0.92±0.31	—

Scanning electron microscopy observation on the antennal sensilla of Ooencyrtus kuvanae

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[1]	JIANG Yuting, LI Chengde, FAN Yongmei. Reports of a new record genus and description of a new species of Encyrtidae (Hymenoptera: Chalcidoidea) from China [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2022, 46(4): 209-211.
[2]	Ye Xiaochun Chen Minzhong Can Xuchua. SEM APPLICATION TO THE RESEARCH OF STRUCTURE OF PAPER [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 1988, 12(03): 107-109.