Scanning electron microscopy observation on the antennal sensilla of Ooencyrtus kuvanae

WANG Jianjun, WANG Jingxian, LUAN Qingshu, DU Hui, JIANG Xu, DONG Lili, CAO Chuanwang

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2022, Vol. 46 ›› Issue (3) : 157-164.

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南京林业大学学报（自然科学版）
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JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2022, Vol. 46 ›› Issue (3) : 157-164. DOI: 10.12302/j.issn.1000-2006.202106012

Scanning electron microscopy observation on the antennal sensilla of Ooencyrtus kuvanae

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Abstract

【Objective】The type, number and distribution characteristics of the antennal sensilla of male and female Ooencyrtus kuvanae were determined, to provide a basis for exploring the internal structure, sensing mechanisms and related functions of each type of sensilla.【Method】The antenna structure, length and morphological characteristics of antennal sensilla of O. kuvanae were observed using scanning electron microscopy (SEM). The types of sensilla were identified, the number and location were counted for different types of sensilla on the back and ventral surfaces of the antennae. The length of the antenna and the length, width and number of the same type of sensilla between the female and male were compared using an independent sample t test. The differences in the number and distribution density of the sensilla on different antennal segments were compared between the female and male.【Result】The antennae of O. kuvanae were geniculate and the antennae length of the female (806.79 ± 4.30) μm was larger than that of the male (781.13 ± 7.13) μm. The flagellum of the female had 9 segments, including 6 funicles and 3 clavae. The flagellum of the male comprised 7 segments, including 6 funicles and 1 clava. There were 7 types of sensilla on the antennae of both the female and male, including trichomes sensilla (TS), chaetica sensilla (ChS), campullacea sensilla (CaS), placodea sensilla (PS), mammilliformia sensilla (MS), Böhm bristles (BB) and finger-like sensilla (FS). The TS and FS were divided into typeⅠand typeⅡ. The numbers of TS on the female and male were the largest and the most widely distributed. There was no significant difference in the numbers of TSⅠand TSⅡbetween the female and male. The numbers of ChS and FSⅠon the female were significantly greater than that on the male. The numbers of BB and FSⅡon the female were significantly less than that on the male. There was no significant difference in the numbers of PS, CaS and MS between the female and male. The types, number and density of sensilla on the antennae increased from the base to the end.【Conclusion】The total quantity of antennal sensilla on the female and male were similar, but the number, size and distribution of each type of sensilla were different, with pronounced sexual dimorphism. The CaS was found in Encyrtidae. The flagellum of O. kuvanae may play a more important role in the process of host localization, recognition and oviposition.

Key words

Ooencyrtus kuvanae / parasitoid / Lymantria dispar / antennal sensilla / scanning electron microscopy (SEM) / sexual dimorphism

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WANG Jianjun , WANG Jingxian , LUAN Qingshu , et al . Scanning electron microscopy observation on the antennal sensilla of Ooencyrtus kuvanae[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2022, 46(3): 157-164 https://doi.org/10.12302/j.issn.1000-2006.202106012

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