Effects of exogenous signal substances on growth and photosynthetic characteristics of Santalum album seedlings

doi:10.12302/j.issn.1000-2006.202207037

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2022, Vol. 46 ›› Issue (6): 271-278.doi: 10.12302/j.issn.1000-2006.202207037

Special Issue: 南京林业大学120周年校庆特刊

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Effects of exogenous signal substances on growth and photosynthetic characteristics of Santalum album seedlings

LI Zhenshuang¹^,²(), WANG Qian¹, ZHU Yuan¹, YANG Fucheng¹, LIANG Junfeng¹, LU Junkun¹^,^*()

1. Research Institute of Tropical Forestry, Chinese Academy of Forestry, Key Laboratory of National Forestry and Grassland Administration on Tropical Forestry Research, Guangzhou 510520, China
2. Nanjing Forestry University, Nanjing 210037, China

Received:2022-07-25 Revised:2022-09-17 Online:2022-11-30 Published:2022-11-24
Contact: LU Junkun E-mail:zhenshuangli@caf.ac.cn;junkunlu@caf.ac.cn

Abstract

Abstract:

【Objective】Root exudates play an important role in the plant interspecific relationship. The effects of host root exudates and chemicals on growth, root development, nutrient accumulation and photosynthetic characteristics of hemiparasitie Santalum album seedlings were studied. The superior host root exudates or several chemicals were screened to provide a scientific guidance for cultivating S. album seedlings.【Method】Under greenhouse conditions, we performed the pot experiment to determine whether root exudates (Acacia confusa, Bischofia polycarpa, Dalbergia odorifera, Dracontomelon duperrereanum and S. album) and haustorium-inducing chemicals (2,6-dimethoxy-p-benzoquinone (DMBQ), formononetin, quercetin and syringaldehyde) could promote the growth and root development of S. album seedlings. The growth indices (height, diameter and biomass), root morphology (total root length and root surface area), nutrient (N, P, K) contents, and photosynthetic characteristics (SPAD values, photosynthetic rate (P_n), stomatal conductance (G_s), transpiration rate (T_r)) of S. album seedlings were measured after a 3-month treatment.【Result】The height, shoot and root biomass of S. album seedlings exhibited the significantly greater growth when treated with Dalbergia odorifera root exudates, DMBQ and syringaldehyde. The total root length, root surface area and length of thin fine roots were significantly greater in S. album seedlings treated with 4 root exudates (except for B. polycarpa) and 4 chemicals than those of the control treatment. The exogenous signal substance could promote N, P and K absorption of S. album seedlings, especially for Dalbergia odorifera root exudate and DMBQ. Root exudates of Dalbergia odorifera and A. confusa, DMBQ and syringaldehyde induced significantly higher SPAD values. Dalbergia odorifera root exudate and 4 chemicals could induce significantly higher photosynthetic characteristics of S. album seedlings. The correlation analysis shows that there are significant and strong correlations between growth indices, physiology indices and root growth (except for thick fine root and coarse root length). Haustoria number of S. album seedlings are significantly correlated with the total the root length and root surface area, indicating that the haustorium development of S. album is closely related to root growth.【Conclusion】The application of root exudates of superior host and/or haustorium-inducing chemicals could effectively promote root development, nutrient absorption, and photoautotrophic capability of S. album seedlings. Our results provide insights into S. album nursery in south China.

Key words: exogenous signal substance, Santalum album, root exudates, root morphology, photosynthetic characteristics

CLC Number:

S723

LI Zhenshuang, WANG Qian, ZHU Yuan, YANG Fucheng, LIANG Junfeng, LU Junkun. Effects of exogenous signal substances on growth and photosynthetic characteristics of Santalum album seedlings[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2022, 46(6): 271-278.

Figures/Tables 5

Table 1

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类别 item	处理 treatmant	苗高/cm height	地径/mm diameter	地上生物量/g shoot biomass	地下生物量/g root biomass	总根长/cm total root length	根表面积/ cm² root surface area	根长/cm root length
类别 item	处理 treatmant	苗高/cm height	地径/mm diameter	地上生物量/g shoot biomass	地下生物量/g root biomass	总根长/cm total root length	根表面积/ cm² root surface area	小细根 thin fine root	粗细根 thick fine root	粗根 coarse root
	台湾相思 A. confusa(Ac)	22.4±0.66 bc	2.01±0.05 b	0.81±0.09 ab	0.13±0.02 bc	257±28 bc	32.7±2.75 b	245±27 bc	7.54±0.91 bc	5.08±0.46 ab
	重阳木 B. polycarpa(Bp)	20.6±0.94 c	2.22±0.07 ab	0.80±0.06 ab	0.12±0.03 bc	205±39 cd	29.6±3.97 bc	192±38 cd	7.72±1.38 bc	5.02±0.75 ab
根系分泌物 root exudates	人面子 Dracontomelon duperreranum(Dd)	21.1±0.89 c	2.24±0.12 ab	0.85±0.10 a	0.12±0.01 bc	243±27 bc	32.6±2.74 b	228±27 bc	9.90±1.12 abc	5.54±0.52 a
	降香黄檀 Dalbergia odorifera(Do)	25.6±0.76 a	2.43±0.09 a	1.02±0.09 a	0.18±0.02 a	385±26 a	50.1±3.48 a	366±25 a	12.36±1.05 a	6.32±0.79 a
	檀香 S. album(Sa)	22.0±1.16 bc	2.25±0.11 ab	0.81±0.06 ab	0.12±0.01 bc	288±13 b	36.8±1.97 b	271±14 b	10.49±1.60 ab	6.45±0.71 a
	2,6-二甲氧基对苯醌 DMBQ	25.8±1.32 a	2.54±0.11 a	0.99±0.07 a	0.17±0.02 ab	390±17 a	49.3±2.30 a	372±16 a	11.93±1.07 a	6.29±0.68 a
化学物质 chemicals	芒柄花素 formononetin(Fo)	22.4±0.66 bc	2.36±0.09 a	0.86±0.07 a	0.12±0.01 bc	278±18 bc	34.2±2.00 b	264±18 bc	9.27±0.98 abc	4.55±0.38 ab
	槲皮素 quercetin(Qu)	24.3±1.02 ab	2.49±0.07 a	0.80±0.07 ab	0.12±0.01 bc	279±26 bc	35.7±3.15 b	264±25 bc	9.64±1.05 abc	5.56±0.63 a
	丁香醛 syringaldehyde(Sy)	25.3±0.87 a	2.44±0.12 a	0.89±0.07 a	0.14±0.01 ab	278±20 bc	35.8±2.33 b	264±20 bc	8.83±0.83 abc	5.57±0.61 a
对照 CK	水 water	20.2±0.57 c	2.30±0.13 ab	0.58±0.05 b	0.08±0.01 c	163±9 d	22.0±0.97 c	153±10 d	6.51±0.62 c	3.43±0.28 b

Effects of exogenous signal substances on growth and photosynthetic characteristics of Santalum album seedlings

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