Softwood cutting technology for Acer truncatum and physiological and biochemical analysis during rooting process

doi:10.12302/j.issn.1000-2006.202208015

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2024, Vol. 48 ›› Issue (5): 123-130.doi: 10.12302/j.issn.1000-2006.202208015

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Softwood cutting technology for Acer truncatum and physiological and biochemical analysis during rooting process

WANG Yuxiao¹(), ZHANG Bin², MA Qiuyue³^,^*(), FU Wei¹, KANG Zhen⁴, ZHU Changhong⁴, LI Shuxian¹

1. Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry and Grassland, Nanjing Forestry University, Nanjing 210037, China
2. The Third Geological and Mineral Exploration Institute of Gansu Bureau of Geology and Mineral Resources,Lanzhou 730050,China
3. Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
4. Forestry Science and Technology Spreading Station in Xiangyang, Xiangyang 441022, China

Received:2022-08-08 Revised:2022-10-24 Online:2024-09-30 Published:2024-10-03
Contact: MA Qiuyue E-mail:wangyux1997@163.com;yue.870808@163.com

Abstract

Abstract:

【Objective】 In order to investigate the effects of different treatments on the rooting of softwood cuttings of Acer truncatum and find the best treatment method. We analyzed the relationship between changes in nutrient content, oxidase activity, and rooting of cuttings, and preliminarily explored the rooting mechanism of A. truncatum. 【Method】 Using rejuvenated A. truncatum trees and three-year-old A. truncatum mother trees as study materials, we conducted an analysis of the effects of growth regulators [indole-3-butyric acid (IBA) and 1-naphthaleneacetic acid (NAA) and a rooting solution], cutting positions (upper and lower parts), rejuvenation, and cutting time (June and September) on the rooting of A. truncatum. Furthermore, we examined the rate of root growth, callus formation, number of roots, root length, and other indicators of root development. Additionally, changes in nutrient contents (soluble sugar and soluble protein) and changes in oxidase activity [peroxidase (POD), polyphenol oxidase (PPO), and indole-3-acetic acid oxidase (IAAO)] were measured during the rooting process. 【Result】 The optimum IBA and NAA concentrations were 500 and 800 mg/L respectively, and the rooting rates were 59.3% and 52.7%, respectively. The optimal concentration for the rooting solution was a 1.43% volume fraction (70-fold) dilution, which achieved a rooting rate of 43.9%. Significant differences were observed among the three treatments, with all rooting indexes of cuttings treated with growth regulators performed better than those of the control. Additionally, the rooting effect of cuttings in June was better than that in September; the rooting effect of rejuvenated mother trees was better than that of 3-year-old trees; and the rooting effect of upper cuttings was better than that of lower cuttings. There were substantial differences observed following these treatments. During the rooting of the cuttings, the changes in the soluble sugar content following the treatment displayed a decreasing trend followed by an increasing trend. The soluble protein content, POD activity, and PPO activity initially increased and then decreased. However, the IAAO activity displayed a trend of decrease-increase-decrease. The control group exhibited a similar changing trend to that of the treatment group, but the peak time for the treatment group was 5-10 days earlier than that of the control. 【Conclusion】 The adventitious root formation of A. truncatum was categorized as a mixed rooting type. When the upper cuttings of rejuvenated mother trees were treated with IBA 500 mg/L, all rooting indexes were optimized, and the rooting rate peaked at 81.3%. Growth regulator treatment can improve the metabolism rate of nutrients and the synthesis process of related oxidase, and has a facilitative effect on the rooting of cuttings.

Key words: Acer truncatum, growth regulator, softwood cutting, nutrient, oxidase

CLC Number:

S792.35

WANG Yuxiao, ZHANG Bin, MA Qiuyue, FU Wei, KANG Zhen, ZHU Changhong, LI Shuxian. Softwood cutting technology for Acer truncatum and physiological and biochemical analysis during rooting process[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2024, 48(5): 123-130.

Figures/Tables 7

Table 1

Fig. 1

Table 2

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Table 4

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Fig. 3

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生长调节剂 growth regulation	用量 concentration	处理时间 treatment time
对照CK	清水	—
吲哚丁酸IBA	300、500、700 mg/L	速蘸10 s
萘乙酸NAA	800、1 000、1 200 mg/L	速蘸10 s
生根液 rooting solution	体积分数3.33%、2.00%、1.43% (稀释30、50、70倍)	浸泡30 min

处理 treatment	用量 concentration	愈伤率/% callus rate	生根率/% rooting rate	平均根长/cm average root length	平均根数/条 mean number	根系指数 rooting index
	CK1	35.0±1.7 e	32.2±1.9 e	8.8±0.0 cd	1.7±0.1 cd	4.8±0.2 e
萘乙酸 NAA	800 mg/L	53.3±3.3 c	52.7±3.1 b	8.5±0.7 cd	1.9±0.1 abc	8.5±0.2 bc
	1 000 mg/L	64.8±1.8 a	46.4±3.4 cd	9.3±0.7 bc	2.1±0.1 a	9.1±1.0 b
	1 200 mg/L	58.4±2.4 b	44.0±4.0 d	7.9±0.3 d	1.8±0.1 bcd	5.8±0.3 de
吲哚丁酸 IBA	300 mg/L	46.2±2.7 d	50.6±4.2 bc	10.1±0.4 b	2.1±0.2 a	10.9±1.9 a
	500 mg/L	42.7±3.1 d	59.3±4.1 a	8.8±0.1 cd	1.8±0.1 bcd	9.6±0.6 ab
	700 mg/L	44.1±2.3 d	50.4±2.7 bc	9.1±0.0 c	2.1±0.1 a	9.7±0.2 ab
	CK2	45.0±1.7 d	30.3±2.5 e	8.4±0.2 cd	1.8±0.1 d	4.5±0.4 e
生根液体积分数 rooting solution valume fraction	3.33%(30倍)	47.3±4.4 d	36.1±2.8 e	11.7±1.2 a	1.7±0.1 d	7.0±1.3 cd
	2.00%(50倍)	62.7±2.5 ab	35.2±2.0 de	8.5±0.1 cd	2.0±0.2 ab	5.9±0.8 de
	1.43%(70倍)	65.0±5.0 a	43.9±3.5 d	10.1±0.6 b	2.1±0.2 a	9.3±1.1 ab

扦插时间 cutting time	愈伤率/% callus rate	生根率/% rooting rate	平均根长/cm average root length	平均根数/个 mean elements	根系指数 rooting index
6月16日	57.0±2.0 a	65.3±0.6 a	12.7±1.2 a	1.1±0.2 a	9.1±0.4 a
9月14日	43.0±3.0 b	47.7±2.1 b	8.8±0.1 b	0.6±0.0 b	2.5±0.1 b

采穗母树年龄 parent stock age	部位 position	愈伤率/% callus rate	生根率/% rooting rate	平均根长/cm average root length	平均根数/个 mean elements	根系指数 rooting index
幼化 rejuvenation	下部	66.3±1.5 b	72.3±2.1 b	12.3±1.1 a	1.3±0.1 b	11.6±0.3 b
	上部	74.3±3.5 a	81.3±2.1 a	13.0±1.0 a	1.6±0.1 a	16.9±0.3 a
3年生 three-year old	下部	56.7±1.5 c	45.7±2.0 d	8.3±0.6 b	1.1±0.1 c	4.2±0.2 d
	上部	60.7±0.6 c	64.3±1.2 c	9.3±0.6 b	1.1±0.2 c	6.6±0.2 c

Softwood cutting technology for Acer truncatum and physiological and biochemical analysis during rooting process

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