Analyzing ultrastructure of Pcythropus yasumatsui (Coleoptera: Curculionida) antennal sensilla by the scanning electron microscope

doi:10.12302/j.issn.1000-2006.202312004

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2024, Vol. 48 ›› Issue (6): 245-251.doi: 10.12302/j.issn.1000-2006.202312004

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Analyzing ultrastructure of Pcythropus yasumatsui (Coleoptera: Curculionida) antennal sensilla by the scanning electron microscope

YAN Xiongfei(), YANG Liuji, LIU Yonghua, LI Gang, FENG Jiangshuai

College of Life Sciences, Yulin University, Yulin 719000, China

Received:2023-12-02 Revised:2024-08-20 Online:2024-11-30 Published:2024-12-10

Abstract

Abstract:

【Objective】Pcythropus yasumatsui(Coleoptera: Curculionida) is a type of weevil, which is an important pest of Ziziphus jujuba, causing serious economic losses to the jujube area in northern Shaanxi. Studying the ultrastructure of the antennal sensilla of the weevil is helpful to understand the chemical communication mechanism of the weevil’s searching host, mating and aggregation behavior.【Method】P. yasumatsui adults were collected from jujube orchard in Jia County, Yulin City, Shaanxi Province. The antennae were cut off and fixed, cleaned and dried in turn. The morphological structure of the antennae was observed by optical microscope. Then, the antennae were sprayed with gold by ion sputtering instrument. The ultrastructure and distribution of the sensilla on the antennae were observed and analyzed by the scanning electron microscope. Finally, the types of sensilla were classified and named according to the classical naming method.【Result】The female and male adults of P. yasumatsui were both geniculate antenna, with a total of three segments, one segment of scape,one segment of pedicel and one segment of flagellum. The flagellum was divided into seven subsegments, and the 7th subsegment of flagellum was enlarged and showed a flame shape. There were seven types and 13 kinds of sensilla and culticular pores on the antennae of female and male adults, including four kinds of sensilla chaetica, one kind of sensilla squamiformia, three kinds of sensilla trichodea, two kinds of sensilla basiconica, one kind of sensilla gemmiformium, one kind of sensilla campaniformia and Böhm bristles. Among them, the number of sensilla chaetica was the largest, followed by sensilla squamiformia. There was no difference in the types of antennal sensilla between male and female adults. However, the number of females’ antennal sensilla was more than that of males.【Conclusion】The antennae of P. yasunatsui have a total of seven types and 13 kinds of sensilla, sensilla chaetica Ⅳ, sensilla basiconica Ⅱ, sensilla trichodea Ⅱ, sensilla campaniforme and Böuhm’s bristles were newly discovered.They play an important role in host localization, courtship and aggregation, and could be used as a reference for further research on the chemical ecological regulation technology of P. yasumatsui.

Key words: Pcythropus yasumatsui, antenna, sensilla, scanning electron microscope, ultrastructure

CLC Number:

S763

YAN Xiongfei, YANG Liuji, LIU Yonghua, LI Gang, FENG Jiangshuai. Analyzing ultrastructure of Pcythropus yasumatsui (Coleoptera: Curculionida) antennal sensilla by the scanning electron microscope[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2024, 48(6): 245-251.

Figures/Tables 5

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References 35

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性别 gender	柄节长度/μm scape length	梗节长度/μm pedicel length	鞭节各亚节长度/μm subsegments length of flagllum
性别 gender	柄节长度/μm scape length	梗节长度/μm pedicel length	F1	F2	F3	F4	F5	F6	F7
雌虫femle	543.9±20.4	164.2±5.4	93.7±3.8	74.8±6.2	69.9±1.9	62.3±3.0	65.3±2.2	66.3±2.0	260.1±7.1
雄虫male	590.6±26.3	190.3±6.9	90.8±6.1	73.2±2.3	71.3±3.8	63.2±3.2	62.5±3.8	65.2±2.2	273.6±8.0

类型 type	感器窝形状 socket shape	端部形状 tip shape	感器壁形状 wall shape	整体形状 whole shape	长度/μm length	直径/μm diameter	类型 type	感器窝形状 socket shape	端部形状 tip shape	感器壁形状 wall shape	整体形状 whole shape	长度/μm length	直径/μm diameter
刺形感器SchⅠ	宽阔	急尖	深纹	平直	62.37±2.86	5.38±0.43	毛形感器StⅢ	宽阔或紧实	钝尖	光滑	平直	10.46±0.46	1.89±0.08
刺形感器SchⅡ	宽阔	钝尖	浅纹	弯曲	30.27±1.63	2.51±0.09	蒲姆氏鬃毛Bb	宽阔	急尖	光滑	平直	5.65±0.50	1.63±0.05
刺形感器SchⅢ	宽阔	急尖	深纹	弯曲	33.39±0.89	2.99±0.21	锥形感器SbⅠ	宽阔	钝尖	光滑	平直	5.88±0.37	2.05±0.07
刺形感器SchⅣ	紧实	急尖	深纹	平直	26.03±0.56	2.16±0.08	锥形感器SbⅡ	宽阔	钝尖	光滑	平直	3.71±0.07	1.46±0.04
鳞形感器Ss	宽阔		深纹	平直	36.87±1.45	8.97±0.42	芽孢形感器Sg		急尖	光滑	平直	2.03±0.18	0.97±0.08
毛形感器StⅠ	宽阔或紧实	钝尖或急尖	光滑	弯曲或平直	22.75±1.18	2.03±0.09	钟形感器Sc			光滑	平直	1.50±0.11	1.95±0.06
毛形感器StⅡ	紧实	急尖	光滑	弯曲	17.33±0.72	2.20±0.08

Analyzing ultrastructure of Pcythropus yasumatsui (Coleoptera: Curculionida) antennal sensilla by the scanning electron microscope

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