Accumulation, toxic properties and bioremediation of autotoxic substance in poplar rhizosphere soil

doi:10.3969/j.issn.1000-2006.2013.03.013

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2013, Vol. 37 ›› Issue (03): 71-76.doi: 10.3969/j.issn.1000-2006.2013.03.013

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Accumulation, toxic properties and bioremediation of autotoxic substance in poplar rhizosphere soil

LI Guanxi^1,2,3, WU Xiaoqin^1,3*,YE Jianren^1,3

1.College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037,China;
2.Lianyungang City Academy of Agricultural Sciences, Lianyungang 222006, China;
3.Jiangsu Key Laboratory for Prevention and Management of Invasive Species, Nanjing 210037, China

Online:2013-06-18 Published:2013-06-18

Abstract

Abstract: Accumulation law of autotoxic substance in poplar rhizosphere soil, toxic properties of autotoxic substance on poplar seedlings, bioremediation characteristic of Burkholderia multivorans WS-FJ9 on autotoxicity, and biodegradation characteristic on autotoxic substance were researched. Autotoxic substance of 1-5 growth years poplar rhizosphere soil was determined by HPLC. Hydroponic poplar seedlings were cultured in Hoagland nutrient solution added with different concentrations of autotoxic substance. Taked some of hydroponic poplar seedlings at the 20^th d after treated with autotoxic substance to inoculate B. multivorans WS-FJ9 strain and continued to cultivate. Physiological and biochemical indexes were studied respectively at the 30^th d after treated with autotoxic substance. Biodegradation rate of B. multivorans WS-FJ9 on autotoxic substance was determined. Autotoxic substance of phenolic acids are presented in different tree-age poplar rhizosphere soil. Different levels of autotoxic substance all can inhibit activities of SOD and POD, enhance membrane permeability and MDA content, and reduce roots activity of hydroponic poplar seedlings. On the other hand, the strain of B. multivorans WS-FJ9 can enhance activities of SOD and POD, reduce membrane permeability and MDA content, and enhance roots activity of hydroponic poplar seedlings. The strain of B. multivorans WS-FJ9 has strong biodegradation ability for the five autotoxic substances. And it has the strongest biodegradation ability for p-hydroxybenzoic acid and vanillin with biodegradation rates of 98.73% and 96.32%, respectively.

CLC Number:

S763.19

LI Guanxi, WU Xiaoqin,YE Jianren. Accumulation, toxic properties and bioremediation of autotoxic substance in poplar rhizosphere soil[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2013, 37(03): 71-76.

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Accumulation, toxic properties and bioremediation of autotoxic substance in poplar rhizosphere soil

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