Abstract: This article aimed to determine the effect of salt stress on physiological and biochemical index of 10 provenances of Cupressus lusitanica by measuring seedling height, ground diameter, relative water content, chlorophyll content, relative conductivity, proline content and malonaldehyde(MDA)content under five salinity gradient levels. The results showed that under salt stress, the seedling’s height, ground diameter and relative water content decreased, while proline content and malonaldehyde(MDA)content increased outstandingly. Salt stress affected the height growth more than ground diameter growth. Total content of chlorophyll increased under low level of salt stress and decreased under high level of salt stress. Relative conductivity could not show the real growth condition of Cupressus lusitanica suffering salt stress. Based on subordination function method, the three high salt-resistant provenances(D10,D3 and D4)among ten provenances are obtained.