Changes of carbohydrate content and related enzyme activity during pollen germination of two bulb flowers

GENG Xingmin, QIU Jianyu, WANG Lianggui

Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 2013, Vol. 37 ›› Issue (04) : 34-38.

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南京林业大学学报（自然科学版）
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Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 2013, Vol. 37 ›› Issue (04) : 34-38. DOI: 10.3969/j.issn.1000-2006.2013.04.007

Changes of carbohydrate content and related enzyme activity during pollen germination of two bulb flowers

  • GENG Xingmin, QIU Jianyu, WANG Lianggui
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Abstract

Lilium pollen differed with Gladiolus pollens on the dependence of pollen germination and pollen tube growth on sugar sources. To clearly know the mechanism of pollen germination of Lilium and Gladiolus, the content of carbohydrates and related enzyme activity of germinating pollen were determined based on qualitative analysis of pollen carbohydrates. Starch was detected in Lilium pollen, but not in Gladiolus pollen. Contents of sucrose, glucose and fructose in Lilium pollen were significantly higher than that in Gladiolus pollen. In addition, germination rate of both kinds of pollen was higher in PEG4000+H3BO3, and Lilium pollen could germinate in culture medium only containing PEG, but Gladiolus pollen could not. When the pollens were cultured in PEG4000+H3BO3, the changes of amylase activity in germinating pollen were not significant, but the amylase activity of Lilium pollen was higher than that of Gladiolus pollen. The changes of invertase and lactase activity in germinating pollen were more significant, and it decreased rapidly at the beginning of pollen culture. The changes of carbohydrate content were very significant, especially glucose. With the rapid growth of pollen tube, glucose content decreased. The difference of pollen germination rate in PEG culture medium and the dependence of pollen germination on sugar sources between Lilium and Gladiolus pollen may be related to the differences of carbohydrate type, content and enzyme activity in nature and germinating pollen.

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GENG Xingmin, QIU Jianyu, WANG Lianggui. Changes of carbohydrate content and related enzyme activity during pollen germination of two bulb flowers[J]. Journal of Nanjing Forestry University (Natural Sciences Edition）. 2013, 37(04): 34-38 https://doi.org/10.3969/j.issn.1000-2006.2013.04.007

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