Abstract: The effects of plant growth regulators on the adventitious bud proliferation and rooting in Camellia oleifera were studied by using new shoot (with buds) and leaf as explants to establish a fast reproductive system. And the effects of different medium on survival rate of transplanted tissue culture seedlings were also investigated. The results showed that:(1) The highest frequency of shoot formation was obtained when explants (stem with buds) were cultured on 1/2MS+NAA(0.5 mg/L)+6-BA(1.0 mg/L)+ZT(0.5 mg/L). The inducement rate of valid buds reached to 745%. The regenerated shoot from leaf was obtained when cultured on 1/2MS+TDZ (1.0 mg/L)+IAA(0.1 mg/L)+6-BA(2.0 mg/L) for 7 weeks. (2) Regenerated shoot were cut as several cluster or single plantlet and transferred on 1/2MS+6-BA(1.0 mg/L)+NAA(0.05 mg/L) for shoot propagation and growth, which the multiplication coefficient was more than 417. (3) 883% of rooting rate, 372 cm of average root length, and 8 roots of average plantlet were observed when cultured in 1/2 MS for 50 days, after shoot was dipped in 1 g/L IBA for 5 second. (4) The rooted plantlets were acclimatized on mixture of peat and vermiculite(1∶1), which survival rate was 82.5%.