Development of simple sequence repeats base on pine wood nematode 
(Bursaphelenchus xylophilus)genome sequence

doi:10.3969/j.issn.1000-2006.2014.02.008

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2014, Vol. 38 ›› Issue (02): 36-42.doi: 10.3969/j.issn.1000-2006.2014.02.008

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Development of simple sequence repeats base on pine wood nematode (Bursaphelenchus xylophilus)genome sequence

XU Junrong, WU Xiaoqin^*, LIU Yun, YE Jianren

Jiangsu Key Laboratory for Prevention and Management of Invasive Species,College of Forest Resources and Enviroment,Nanjing Forestry University,Nanjing 210037,China

Online:2014-03-10 Published:2014-03-10

Abstract

Abstract: To develop more stable molecular marker, SSR marker base on pine wood nematode(Bursaphelenchus xylophilus)whole genome sequences was discussed for researching the relationship of population genetic and travel route accurately. Ninety-five SSR loci were searched from a total of 10 432 DNA fragments of B.xylophilus by MISA. In the gSSRs, the dinucleotide repeat motifs were the most abundant(66.3%). Thirty-six pairs of primers were designed, and verified with one peace of B.xylophilus DNA pooling sample(46 DNA samples of isolates from different geographic origin contained)for PCR. The products detected by QIAxcel automatic gel electrophoresis analysis system showed that 17 pairs of primers had potential polymorphism. Further, nine pairs of the primers showed polymorphism, which tested by monoclonal sequencing and spliced by BioEdit, and which provided foundation for studying population genetic of B.xylophilus.

CLC Number:

Q78
S763.16

XU Junrong, WU Xiaoqin, LIU Yun, YE Jianren. Development of simple sequence repeats base on pine wood nematode (Bursaphelenchus xylophilus)genome sequence[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2014, 38(02): 36-42.

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Development of simple sequence repeats base on pine wood nematode (Bursaphelenchus xylophilus)genome sequence

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