Laboratory decomposition research of Lophodermium on the litter needles from Pinus armandii

doi:10.3969/j.issn.1000-2006.2014.03.015

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2014, Vol. 38 ›› Issue (03): 77-82.doi: 10.3969/j.issn.1000-2006.2014.03.015

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Laboratory decomposition research of Lophodermium on the litter needles from Pinus armandii

YANG Chunlin,XU Xiulan,TIAN Sha,WEN Zilan,LIU Yinggao^*,JIANG Xinhua,ZHANG Yan,LIU Huitao

College of Forestry, Sichuan Agricultural University, Ya’an 625014, China

Online:2014-05-15 Published:2014-05-15

Abstract

Abstract: In order to study the saprophytic ability of Lophodermium spp. and mechanism of each cellulase produced in the process of decomposing litters, newly litter needles from Pinus armandii were used as the decomposition material to conduct a decomposition experiment in pure culture. Five species of Lophodermium(L. conigenum, L. pinastri, L. kumaunicum, L. sichuanense, L. erlangshanense)were cultivated, in which the needles were used as the sole carbon source. The weight loss rate of the needles and the cellulase enzyme activities(Filter paper activity(FPA), Endoglucanase(EG), Cellobiohydrolase(CBH), β-glucosidase(BGL))were determined in the test. The correlation analysis showed that there was a certain synergistic effect among EG, CBH and BGL(except L. pinastri and L. erlangshanense), especially among CBH and EG. Cellulose decomposition was restricted by each enzyme activity, the litter needles could be decomposed faster with higher synergistic effect and high enzyme activity. The mass loss rates caused by five Lophodermium spp. was between 8.5%-11.8%, and which declined in the following order: L. pinastri>L. sichuanense>L. kumaunicum>L. conigenum>L. erlangshanense. The five kinds of Lophodermium spp. can produce cellulase, and they have different levels of synergistic effect among EG, CBH and BGL. They all had certain ability to decompose the needles, and they are facultative parasites, but they have weak saprophytism.

CLC Number:

S154

YANG Chunlin,XU Xiulan,TIAN Sha,WEN Zilan,LIU Yinggao,JIANG Xinhua,ZHANG Yan,LIU Huitao. Laboratory decomposition research of Lophodermium on the litter needles from Pinus armandii[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2014, 38(03): 77-82.

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Laboratory decomposition research of Lophodermium on the litter needles from Pinus armandii

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