Abstract: In order to obtain a good decolorization efficiency of dye wastewater,we fixed spore laccase from Bacillus amyloliquefaciens LC03 in seaweed acid calcium gel and determined its optimum temperature and pH and its operational stability.Then we studied the decolorization of four single dyes,including remazol brilliant blue R(RBBR),reactive black 5,indigo carmine and crystal violet, and investigated the effects of immobilized laccase-mediators with laccase mediators added on single dye to stimulate dye wastewater.Experiments showed that the optimum temperature was 65 ℃,the optimum pH was 6.2 and after 10 cycles of operation the immobilized enzyme remained over 80% of its initial activity. The immobilized pellets had a significant effect on decolorization of CV and more than 80% of CV was decolorized after 1 hour,while it also played some role in the decolorization of other three single dyes. Laccase mediators had a promoting effect on the decolorization of dyes especially on the IC,RBBR and RB5 and with the mediators concentration rising,the rate of decolorization increased. The immobilized pellets enhanced laccase repeated utilization ratio and increased the stability of laccase. And under the acid, alkaline, and neutral conditions, immobilizod laccase mediators had a good decolorizing effect on single dye and simulated dye wastewater which indicating potential application of this immobilized laccase-mediators in dye wastewater treatment.