JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2015, Vol. 39 ›› Issue (01): 17-21.doi: 10.3969/j.issn.1000-2006.2015.01.004

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Induction and proliferation of embryogenic callus in nematode-resistant Pinus densiflora

WU Jing, ZHU Lihua,XU Jianxiu, WU Xiaoqin, YE Jianren*   

  1. Collaborative Innovation Center of Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China
  • Online:2015-01-31 Published:2015-01-31

Abstract: Embryogenic callus was induced from immature embryos of nematode-resistant Pinus densiflora. Factors affecting callus initiation were investigated. The results showed that successful initiation of embryogenic callus depended heavily on embryo developmental stage, the combination of auxin and cytokinins, and carbon source. The initiation of embryogenic callus worked best when immature mega gametophytes developed at stage 2-4 were used as starting material. The optimal combination of hormone was 2 mg /L 2,4-D and 1 mg /L 6-BA with the induction rate of 5%. Maltose was better than sucrose and glucose. Further proliferation of embryogenic callus was achieved by transfering explants to a medium with reduced levels of plant growth regulators.

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