JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2015, Vol. 39 ›› Issue (01): 149-154.doi: 10.3969/j.issn.1000-2006.2015.01.027

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Enrichment strategies and prospects of phosphoprotein(phosphopetide)in phosphoproteomics

ZHEN Yan, LI Chunying, LU Ye, SHI Jisen*   

  1. Key Laboratory of Forest Genetics &
    Biotechnology of Ministry of Education, Nanjing Forestry University, Nanjing 210037, China
  • Online:2015-01-31 Published:2015-01-31

Abstract: Protein phosphorylation is an important regulation way in cellular signaling pathways. However, it was difficult to study on phosphoprotein/phosphopetide because of the low abundances, low stoichiometry, and easily to degrade in biological environment. The ideal sample preparation was one of the key steps for protein phosphorylation research. The strategy of phosphoprotein/phosphopetide enrichment with antibody, biotin-tagging approach, phosphopetide enrichment with immobilized metal affinity chromatography, metal oxide affinity chromatography, sequential elution from IMAC, ion exchange chromatography, and the hydrophilic interaction liquid chromatography were discussed in this review, respectively. The importance and application of the phosphorylated proteins/peptides enrichment strategy had been proposed for the further study on regulating factors during cellular signaling transduction pathways.

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