JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2015, Vol. 39 ›› Issue (04): 1-6.doi: 10.3969/j.issn.1000-2006.2015.04.001

Previous Articles     Next Articles

A study on pollen germination and viability in different varieties of Carya illinoensis

ZHU Xuechen,WANG Gaiping*, PENG Fangren,LYU Xin   

  1. College of Forestry, Nanjing Forestry University, Nanjing 210037, China
  • Online:2015-07-31 Published:2015-07-31

Abstract: In order to analyze effects of different culture conditions on pollen vitality, influences of culture time,culture temperature, pH value of culture medium,sugar,boric acid and PEG-4000 concentration on pollen germination were studied by in vitro culture of four different Carya illinoensis cultivars.The results showed that culture time had extremely significant effect on the pollen germination and the pollen length of C. illinoensis. The suitable culture time was 24 h. The culture temperature,pH value,sugar boric acid and PEG-4000 concentrations in liquid medium had obvious influence on pollen viability of the four varieties.The optimum culture temperature for all varieties was 25-30 ℃. The weak acidic environment was favorable for germination and growth of pollens, and the suitable pH value of liquid medium was 5.6.The pollen germination rate and pollen tube length reached their peaks when the sugar concentration was 20%-25%, the concentration of boric acid was over 0.03%; and the concentration of PEG-4000 was over 15%.

CLC Number: