Resistance responses of Castanea mollissima callus to Cp-toxin
 under Cryphonectria parasitica stress

doi:10.3969/j.issn.1000-2006.2015.05.001

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2015, Vol. 39 ›› Issue (05): 1-6.doi: 10.3969/j.issn.1000-2006.2015.05.001

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Resistance responses of Castanea mollissima callus to Cp-toxin under Cryphonectria parasitica stress

HAN Shan^1,2, ZHU Tianhui^1,2*, QIAO Tianmin³, LI Shujiang^1,2, WANG Ju², ZHANG Boyang²

1. College of Forestry,Sichuan Agricultural University,Ya'an 625014,China;
2. Sichuan Provincial Key Laboratory of Forest Protection,Ya'an 625014,China;
3. Sichuan Provincial Key Laboratory of Ecological Forestry Engineering for Upper Reach of Changjiang River,Ya'an 625014,China

Online:2015-10-15 Published:2015-10-15

Abstract

Abstract: In order to clarify the resistance mechanism of different chestnut cultivars to Cp-toxin stress, effects of different concentrations of Cp-toxin from Cryphonectria parasitica on the activities of chitinase, β-1,3-glucanase, the contents of phytoalexin, free proline(Pro)of chestnut callus were analyzed.In this study, a significant correlation was observed between the treatment concentration and chestnut resistance. The results showed that the activities of enzymes increased notably when treated with the low concentrations of Cp-toxin(5-100 μg/mL). When treated with the high concentrations of Cp-toxin(>100 μg/mL),the activities of enzymes and the contents of phytoalexin decreased notably.These data suggested that resistant materials in different varieties increased significantly under the treatment with low concentration of Cp-toxin. This study also indicates that the chitinase, β-1,3-glucanase and phytoalexins were the inherent mechanism for resistance of chestnut callus to toxin stress, and change of free proline content was not an important factor related to the resistance.

CLC Number:

S763

HAN Shan, ZHU Tianhui, QIAO Tianmin, LI Shujiang, WANG Ju, ZHANG Boyang. Resistance responses of Castanea mollissima callus to Cp-toxin under Cryphonectria parasitica stress[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2015, 39(05): 1-6.

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Resistance responses of Castanea mollissima callus to Cp-toxin under Cryphonectria parasitica stress

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