JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2015, Vol. 39 ›› Issue (06): 7-11.doi: 10.3969/j.issn.1000-2006.2015.06.002

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The application of DNA barcoding identification kit technology to Monochamus Dejean

CHEN Mengyi1,2, XU Mei3, QIAN Lu3, AN Yulin3, HAO Dejun1,2*   

  1. 1. Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China;
    2. College of Forestry, Nanjing Forestry University, Nanjing 210037, China;
    3. Jiangsu Entry-exit Inspection and Quarantine Bureau, Nanjing 210001, China
  • Online:2015-11-30 Published:2015-11-30

Abstract: In order to rapidly and accurately identify the Monochamus of wood from Russian, we employed DNA barcode detection kit technology and designed a pair of nested primers based on mitochondrial COⅠ gene(mitochondrial cytochrome oxidase subunit I). Furthermore, COⅠ gene sequence of eight Monocharnus species was amplificated, and uploaded to pest information site. According to differences of arrangement and composition among these species at base sites, species unique bases was got in the corresponding sites, which is named specific site to distinguish this species. In this way, we developed the DNA barcoding identification kit technology to Monochamus species, which will provide a new way for port quarantine identification.

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