Isolation and identification of an antagonistic bacterium against smoke 
tree wilt fungus Verticillium dahliae

doi:10.3969/j.issn.1000-2006.2015.06.004

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2015, Vol. 39 ›› Issue (06): 17-23.doi: 10.3969/j.issn.1000-2006.2015.06.004

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Isolation and identification of an antagonistic bacterium against smoke tree wilt fungus Verticillium dahliae

SUN Yan, YU Dimei, HUANG Huayi, WANG Yonglin, TIAN Chengming^*

Key Laboratory for Silviculture and Conservation of Ministry of Education, College of Forestry, Beijing Forestry University, Beijing 100083, China

Online:2015-11-30 Published:2015-11-30

Abstract

Abstract: In order to isolate and characterize an antagonistic bacterial strain against Verticillium dahliae and Fusarium oxysporum, serial dilution and plate-confrontation methods were used for preliminary screening and selecting antagonistic bacteria from saline-alkali soil. Strain identification was based on morphological and physiological characteristics, and phylogenetic analysis of 16S rDNA sequence. The antagonistic effects of sterile culture filtrates and volatiles of antagonistic bacteria were determined. An antagonistic bacteria, named as C-2-3-2, was isolated and identified as Bacillus subtilis. The results indicated that sterile culture filtrate of the strain exhibited obvious in vitro inhibition against V. dahliae and F. oxysporum, and suppressed the production of microsclerotia of V. dahliae. The volatiles also effectively inhibited the mycelial growth of V. dahliae. In addition, the results indicated that C-2-3-2 could produce ammonia and siderophore, and also could produce cell wall degrading enzymes, such as chitinase, cellulose, protease and β-1,3-glucanase. It indicted that strain C-2-3-2 had high research value and potentials for biological control of Verticillium wilt of Cotinus coggygria.

CLC Number:

S763.1

SUN Yan, YU Dimei, HUANG Huayi, WANG Yonglin, TIAN Chengming. Isolation and identification of an antagonistic bacterium against smoke tree wilt fungus Verticillium dahliae[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2015, 39(06): 17-23.

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Isolation and identification of an antagonistic bacterium against smoke tree wilt fungus Verticillium dahliae

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