Genetic transformation and functional analysis of the SAMT gene in Chimonanthus praecox

doi:10.3969/j.issn.1000-2006.2015.06.006

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2015, Vol. 39 ›› Issue (06): 29-34.doi: 10.3969/j.issn.1000-2006.2015.06.006

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Genetic transformation and functional analysis of the SAMT gene in Chimonanthus praecox

PANG Hongdong^{1, 2}, XIANG Lin³, ZHAO Kaige¹, LI Xiang¹,YANG Nan¹, CHEN Longqing^1*

1.Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, China;
2. Hubei Academy of Forestry, Wuhan 430075, China;
3 Institute of Horticulture, Zhejiang Academy of Agricultural Sciences,Hangzhou 310021, China

Online:2015-11-30 Published:2015-11-30

Abstract

Abstract: In order to investigate the biological function of the Chimonanthus praecox SAMT gene, here we reported that the plant binary expression vector pBI121-CpSAMT was constructed and then the CpSAMT gene was introduced into petunia through Agrobacterium tumefaciens mediated transformation. PCR and RT-PCR was used to test the transgenic plants, and HS-SPME-GC-MS was employed to analyze the components of flower flavor. Results showed that nine independent transgenic lines obtained were positive. Further analysis showed that transgenic plants were indistinguishable from untransformed controls in terms of plant size, leaf morphology, flower color, petal size and flowering time. The analysis of HS-SPME-GC-MS showed that the content of benzaldehyde, phenylethanol, citronellol, citronellyl acetate and phenethyl acetate in flowers of transgenic CpSAMT plants was increased. However, the contents of methyl salicylate and methyl benzoate had not been significantly changed comparing with non-transgenic controls.

CLC Number:

Q78
S685

PANG Hongdong, XIANG Lin, ZHAO Kaige, LI Xiang,YANG Nan, CHEN Longqing. Genetic transformation and functional analysis of the SAMT gene in Chimonanthus praecox[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2015, 39(06): 29-34.

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Genetic transformation and functional analysis of the SAMT gene in Chimonanthus praecox

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