Molecular cloning and expression of a novel β-galactosidase from Bacillus coagulans

ZHENG Zhaojuan, XU Ying, SHI Lei, OUYANG Jia

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2015, Vol. 39 ›› Issue (06) : 35-39.

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JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2015, Vol. 39 ›› Issue (06) : 35-39. DOI: 10.3969/j.issn.1000-2006.2015.06.007

Molecular cloning and expression of a novel β-galactosidase from Bacillus coagulans

  • ZHENG Zhaojuan, XU Ying, SHI Lei, OUYANG Jia*
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Abstract

A novel β-galactosidase gene was cloned from Bacillus coagulans NL01 which had ability to hydrolyze lactose into glucose and galactose in this study. The length of the β-galactosidase gene was 1 998 bp, and its coding sequence showed very low identity with other reported β-galactosidase. The gene was cloned into pETDuet-1 and expressed in Escherichia coli BL21(DE3). The crude enzyme activity was 119.0 μmol/(min·mg), and the purified enzyme activity was 666.4 μmol/(min·mg)after Ni-NTA purification. It was indicated from the analysis results of the hydrolysis product obtained by the purified β-galactosidase that this novel β-galactosidase presented high activity toward lactose conversion into glucose and galactose.

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ZHENG Zhaojuan, XU Ying, SHI Lei, OUYANG Jia. Molecular cloning and expression of a novel β-galactosidase from Bacillus coagulans[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2015, 39(06): 35-39 https://doi.org/10.3969/j.issn.1000-2006.2015.06.007

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