Effect of periodic harvest of enzyme on cellulase production by Trichoderma reesei

doi:10.3969/j.issn.1000-2006.2016.01.014

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2016, Vol. 40 ›› Issue (01): 87-91.doi: 10.3969/j.issn.1000-2006.2016.01.014

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Effect of periodic harvest of enzyme on cellulase production by Trichoderma reesei

BAI Hechao, JIANG Lu, WANG Bucheng, YONG Qiang,YU Shiyuan^*

Key Laboratory of Forest Genetics &
Biotechnology, Ministry of Education, Nanjing Forestry University, Nanjing 210037, China

Online:2016-02-18 Published:2016-02-18

Abstract

Abstract: This paper investigated fed-batch and periodic harvest of enzyme for cellulase production by Trichoderma reesei. The results showed that the best feeding rate was 5.5 g/(L·d)cellulose per day, and the filter paper activity and β-glucosidase were 14.40 U/mL and 1.59 U/mL after 8 days of cultivation. Based on fed-batch, when harvesting enzyme at the 4^th, 6^th, 8^th day, the total filter paper activity improved by 18.14%. When harvesting at the 4^th,7^th day, the total filter paper activity improved by 17.75%. When harvesting at the 5^th,8^th day, the total filter paper activity improved by 27.35%.This paper concluded that periodic harvest of enzyme could improve the total filter paper activity efficiently.

CLC Number:

TQ351
Q814

BAI Hechao, JIANG Lu, WANG Bucheng, YONG Qiang,YU Shiyuan. Effect of periodic harvest of enzyme on cellulase production by Trichoderma reesei[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2016, 40(01): 87-91.

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Effect of periodic harvest of enzyme on cellulase production by Trichoderma reesei

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