Effects of endobacteria on reproduction and virulence of Bursaphelenchus xylophilus

doi:10.3969/j.issn.1000-2006.2016.03.008

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2016, Vol. 40 ›› Issue (03): 47-51.doi: 10.3969/j.issn.1000-2006.2016.03.008

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Effects of endobacteria on reproduction and virulence of Bursaphelenchus xylophilus

HE Longxi1,2, XUE Qi1, WU Xiaoqin1*

1.Co-Innovation Center for Sustainable Forestry in Southern China, Jiangsu Key Laboratory for Pest Prevention and Management of Invasive specie, College of Forestry, Nanjing Forestry University, Nanjing 210037, China;
2. Jiangxi Forest Pest Control and Quarantine Bureau, Nanchang 330038, China

Online:2016-06-18 Published:2016-06-18

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Abstract

Abstract: To investigate the role of endobacterium in the pine wilt disease, the reproduction and virulence of non-parasitic Bursaphelenchus xylophilus, B. xylophilus treated with endobactria and wild-type B. Xylophilus were measured. We found that the spawning number, hatching rate and reproduction number of aseptic B. xylophilus was higher than B. xylophilus treated with bacteria in non-parasitic condition. Extracellular metabolism of endobacterium could inhibit the reproduction of B. xylophilus. After inoculated with 3 years old Pinus massoniana, the reproduction number of nematodes from high to low was as follow:wild-type B. xylophilus>B. xylophilus treated with endobacterium> aseptic B. xylophilus. The pines that inoculated with wild-type B. xylophilus firstly occurred wilting and the course of disease was the shortest in the three different treatments of B. xylophilus. Secondly, the B. xylophilus treated with endobacteria appeared wilting, and the aseptic B. xylophilus appeared lastly. These results indicated that endobacterium exerted different effect on the development of B. xylophilus in non-parasitic and parasitism condition and could enhance the virulence of nematode.

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S763.16

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HE Longxi1,2, XUE Qi1, WU Xiaoqin1*. Effects of endobacteria on reproduction and virulence of Bursaphelenchus xylophilus[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2016, 40(03): 47-51.

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[1]	PAN Yanyi, HUANG Lin, WU Xiaoqin. Bioinformatic and expression analysis of a cathepsin gene Bmcath1 in Bursaphelechus mucronatus [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2015, 39(06): 12-16.
[2]	YUAN Weimin, XIANG Yang, WU Xiaoqin. Endobacteria from different virulence Bursaphelenchus mucronatus [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2015, 39(04): 63-68.
[3]	XU Junrong, WU Xiaoqin, LIU Yun, YE Jianren. Development of simple sequence repeats base on pine wood nematode (Bursaphelenchus xylophilus)genome sequence [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2014, 38(02): 36-42.
[4]	SHI Yanmei, CHEN Fengmao, YE Jianren, FANG Guofei, PAN Hongyang. Classification of parasitic nematodes in pine trees in China [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2012, 36(01): 137-141.