De novo transcriptome sequencing and analysis of carotenoids 
biosynthesis related gene expression in Osmanthus serrulatus

doi:10.3969/j.issn.1000-2006.2016.05.004

De novo transcriptome sequencing and analysis of carotenoids biosynthesis related gene expression in Osmanthus serrulatus

CHEN Lin, LI Longna, DAI Yaping, YANG Guodong

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2016, Vol. 40 ›› Issue (05) : 21-28.

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JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2016, Vol. 40 ›› Issue (05) : 21-28. DOI: 10.3969/j.issn.1000-2006.2016.05.004

De novo transcriptome sequencing and analysis of carotenoids biosynthesis related gene expression in Osmanthus serrulatus

CHEN Lin¹, LI Longna², DAI Yaping¹, YANG Guodong¹

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Abstract

Osmanthus serrulatus is an endemic species of China with high potentiality in the practical application. To investigate the transcriptome profiling of this species, a high throughput RNA sequencing via Illumina HISeq^TM2000 platforms was applied on the flowers and leaf buds of O. serrulatus. Amounting to 16.94 G of clean reads was generated from the sequencing, and then was de novo assembled via trinity. The draft transcriptome assembly consisted of 92 798 unigenes, of which 35 851 unigenes were successfully annotated against the following public databases: Nr, Nt, Swiss-Prot, GO, COG, KEGG and PFAM. The KEGG results showed that a total of 8 779 unigenes could be mapped onto 262 pathways, among which 53 and 335 unigenes involved in the biosynthesis of floral pigment and of fragrance, respectively. In addition, differential gene expression patterns between flowers and leaf buds of O. serrulatus were confirmed by the results of qRT-PCR on 6 genes that are related to the biosynthesis of carotenoid. Our study provides a valuable resource for future studies aimed at identifying the molecular mechanisms of synthesizing flora pigment and fragrance on O. serrulatus.

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CHEN Lin, LI Longna, DAI Yaping, YANG Guodong. De novo transcriptome sequencing and analysis of carotenoids biosynthesis related gene expression in Osmanthus serrulatus[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2016, 40(05): 21-28 https://doi.org/10.3969/j.issn.1000-2006.2016.05.004

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