Interaction between miR164a and its target PeNAC1

WANG Haoran, LI Shuangshuang,LE Lina,KUANG Hualin,HUANG Minren,CHEN Ying

Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 2016, Vol. 40 ›› Issue (05) : 29-33.

PDF(3254435 KB)
南京林业大学学报（自然科学版）
PDF(3254435 KB)
Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 2016, Vol. 40 ›› Issue (05) : 29-33. DOI: 10.3969/j.issn.1000-2006.2016.05.005

Interaction between miR164a and its target PeNAC1

  • WANG Haoran, LI Shuangshuang,LE Lina,KUANG Hualin,HUANG Minren,CHEN Ying*
Author information +
History +

Abstract

miRNA plays a central role in gene regulations. Many research showed that miR164a targets NAC(NAM/ATAF/CUC)genes involved in multiple biological functions, such as plant development, hormones singal transduction and environmental stimuli. To confirm the interaction between micro164a and its predicted target gene PeNAC1 in poplar, the pre-micro164a sequence was cloned from P. trichocarpa genome DNA by PCR, named Ptc-MIR164a. And according to the results of secondary structure analyses with RNA fold(http://rna.tbi.univie.ac.at/), Ptc-MIR164a can form a typical stem-loop structure that suggests it can be processed into mature miRNA164a in vivo. Then combining the principle of luciferase reporter plasmids used in miRNA target detection in animal cell and the transient expression system of poplar protoplasts, we confirmed the interaction between Ptc-MIR164a and PeNAC1 and established a simple and intuitive method for miRNA target detection in plant cell.

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations
WANG Haoran, LI Shuangshuang,LE Lina,KUANG Hualin,HUANG Minren,CHEN Ying. Interaction between miR164a and its target PeNAC1[J]. Journal of Nanjing Forestry University (Natural Sciences Edition）. 2016, 40(05): 29-33 https://doi.org/10.3969/j.issn.1000-2006.2016.05.005

References

[1] Reinhart B J, Weinstein E G, Rhoades M W, et al. MicroRNAs in plants[J]. Genes Dev, 2002, 16(13): 1616-1626. Doi:10.1101/gad.1004402.
[2] Chen X. MicroRNA biogenesis and function in plants[J]. FEBS Lett, 2005, 579(26): 5923-5931. Doi:10.1016/j.febslet.2005.07.071.
[3] Jones-Rhoades M W, Bartel D P, Bartel B. MicroRNAS and their regulatory roles in plants[J]. Annu Rev Plant Biol, 2006, 57: 19-53. Doi:10.1146/annurev.arplant.57.032905.105218.
[4] Mallory A C, Vaucheret H. Functions of microRNAs and related small RNAs in plants[J]. Nat Genet, 2006, 38(S): 31-36. Doi:10.1038/ng1791.
[5] Simon S A, Meyers B C. Small RNA-mediated epigenetic modifications in plants[J]. Curr Opin Plant Biol, 2011, 14(2): 148-155. Doi:10.1016/j.pbi.2010.11.007.
[6] Válóczi A, Várallyay E, Kauppinen S, et al. Spatio-temporal accumulation of microRNAs is highly coordinated in developing plant tissues[J]. Plant J, 2006, 47(1): 140-151. Doi:10.1111/j.1365-313X.2006.02766.x.
[7] Guo H S, Xie Q, Fei J F, et al. MicroRNA directs mRNA cleavage of the transcription factor NAC1 to downregulate auxin signals for Arabidopsis lateral root development[J]. Plant Cell, 2005,17:1376-1386. Doi:10.1105/tpc.105.030841.
[8] Mallory A C, Dugas D V, Bartel D P, et al. MicroRNA regulation of NAC-domain targets is required for proper formation and separation of adjacent embryonic, vegetative, and floral organs[J]. Curr Biol, 2004,14: 1035-1046. Doi:10.1016/j.cub.2004.06.022.
[9] Mao W, Li Z, Xia X, et al. A combined approach of high-throughput sequencing and degradome analysis reveals tissue specific expression of microRNAs and their targets in cucumber[J]. PLoS One, 2012,7(3): e33040. Doi:10.1371/journal.pone.0033040.
[10] Lee J Y, Kim S, Hwang D W, et al. Development of a Dual-luciferase reporter system for in vivo visualization of microRNA biogenesis and posttranscriptional regulation[J]. J Nucl Med. 2008, 49(2):285-294. Doi:10.2967/jnumed.107.042507.
[11] Tan B, Xu M, Chen Y, et al. Transient expression for functional gene analysis using Populus protoplasts[J]. Plant Cell Tiss Organ Cult, 2013, 114(1): 11-18. Doi:10.1007/s11240-013-0299-x.
[12] 王浩然,邵志龙,朱燕宇,等. '南林895'杨PdNAC1 基因克隆及蛋白的亚细胞定位[J].南京林业大学学报(自然科学版),2015,39(3):50-54. DOI:10.3969/j.issn.1000-2006.2015.03.010. Wang H R,Shao Z L,Zhu Y Y,et al. Cloning and sub-cellular localization of PdNAC 1 in poplar ‘Nanlin895'[J]. Journal of Nanjing Forestry University(Natural Sciences Edition), 2015,39(3):50-54.
[13] Llave C, Xie Z, Kasschau K D, et al. Cleavage of Scarecrow-like mRNA targets directed by a class of Arabidopsis miRNA[J]. Science, 2002, 297(5589): 2053-2056. Doi:10.1126/science.1076311.
[14] Laufs P, Peaucelle A, Morin H, et al. MicroRNA regulation of the CUC genes is required for boundary size control in Arabidopsis meristems[J]. Development, 2004, 131(17): 4311-4322. Doi:10.1242/dev.01320.
[15] Fang Y, Xie K, Xiong L. Conserved miR164-targeted NAC genes negatively regulate drought resistance in rice[J]. J Exp Bot, 2014, 65(8): 2119-2135. Doi:10.1093/jxb/eru072.
[16] 曹学武, 陈正堂. miRNA靶基因实验验证的研究进展[J]. 重庆医学, 2009, 38(2): 211-213. Doi:10.3969/j.issn.1671-8348.2009.02.047.
PDF(3254435 KB)

Accesses

Citation

Detail
Sections
Recommended
The full text is translated into English by AI, aiming to facilitate reading and comprehension. The core content is subject to the explanation in Chinese.

Author

Reader

Reviewer

www.nldxb.njfu.edu.cn

Edited ＆ Published by Editorial Department of Nanjing Forestry University(Natural Sciences Edition)
Address： No.159 Longpan Road,Nanjing 210037 Jiangsu,P.R.China
E-mail ：xuebao@njfu.edu.cn , xuebao@njfu.com.cn
Telephone +86-25-85428247,85427076
Distributed by China International Book Trading Corporation P.O. Box 399, Beijing ,P.R. China

/

〈 〉