Interaction between miR164a and its target PeNAC1

doi:10.3969/j.issn.1000-2006.2016.05.005

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2016, Vol. 59 ›› Issue (05): 29-33.doi: 10.3969/j.issn.1000-2006.2016.05.005

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Interaction between miR164a and its target PeNAC1

WANG Haoran, LI Shuangshuang,LE Lina,KUANG Hualin,HUANG Minren,CHEN Ying^*

Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Poplar Germplasm Nursery, Jiangsu Provincial Platform for Conservation and Utilizations for Agricultural Germplasm, Nanjing 210037, China

Online:2016-10-18 Published:2016-10-18

Abstract

Abstract: miRNA plays a central role in gene regulations. Many research showed that miR164a targets NAC(NAM/ATAF/CUC)genes involved in multiple biological functions, such as plant development, hormones singal transduction and environmental stimuli. To confirm the interaction between micro164a and its predicted target gene PeNAC1 in poplar, the pre-micro164a sequence was cloned from P. trichocarpa genome DNA by PCR, named Ptc-MIR164a. And according to the results of secondary structure analyses with RNA fold(http://rna.tbi.univie.ac.at/), Ptc-MIR164a can form a typical stem-loop structure that suggests it can be processed into mature miRNA164a in vivo. Then combining the principle of luciferase reporter plasmids used in miRNA target detection in animal cell and the transient expression system of poplar protoplasts, we confirmed the interaction between Ptc-MIR164a and PeNAC1 and established a simple and intuitive method for miRNA target detection in plant cell.

CLC Number:

S722
Q343

WANG Haoran, LI Shuangshuang,LE Lina,KUANG Hualin,HUANG Minren,CHEN Ying. Interaction between miR164a and its target PeNAC1[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2016, 59(05): 29-33.

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Interaction between miR164a and its target PeNAC1

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