SCAR markers-based molecular identification of 20 
flowering cherries(Cerasus) cultivars

doi:10.3969/j.issn.1000-2006.2016.05.006

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2016, Vol. 59 ›› Issue (05): 34-40.doi: 10.3969/j.issn.1000-2006.2016.05.006

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SCAR markers-based molecular identification of 20 flowering cherries(Cerasus) cultivars

ZHAO Qingjie^1,2, LI Haibo¹, QU Yan², WANG Qinghua³, GUO Jia⁴, XU Liang^{1 Symbolj@@}

1.Zhejiang Forestry Academy, Zhejiang Provincial Key Laboratory of Biological and Chemical Utilization of Forest Resource, Hangzhou 310023, China;
2. School of Landscape, Southwest Forestry University, Kunming 650224, China;
3. East Lake Scenic Area of Wuhan, Wuhan 430074, China;
4. Zhejiang Chengbang Landscape Architecture Company, Hangzhou 310008, China

Online:2016-10-18 Published:2016-10-18

Abstract

Abstract: In the present study, 20 cultivars of flowering cherries(Cerasus), which were widely cultivated in gardens, were identified by developing specific sequence characterized amplified region(SCAR)markers from amplified polymorphic DNA(SAPD)and sequence-related amplified polymorphism(SRAP)techniques. Forty-six polymorphic bands(33 from SAPD and 13 from SRAP)among the 20 studied cultivars were obtained by using 4 SAPD primers and 96 SRAP primers. Fourteen(10 from SAPD and 4 from SRAP)of 46 polymorphic bands were converted into 14 stable and reproducible SCAR markers by designing specific SCAR primers from these sequenced polymorphic bands and by verifying PCR amplification with SCAR primers. The 14 SCAR markers was distributed in 20 different Cerasus cultivars. The cultivars C. serrulata var. lannesiana ‘Albo-rosea', C. serrulata var. lannesiana ‘Superba', C. serrulata var. lannesiana ‘Gioiko', C. serrulata var. lannesiana ‘Kanzan', C. serrulata var. lannesiana ‘Ojochin' and C. serrulata var. lannesiana ‘Asahiyama' possess only one SCAR marker, C.serrulata var. lannesiana ‘Grandifora', Cerasus × sieboldii ‘Beni-yutaka', Cerasus × yedoensis ‘Sakuyahime', C.serrulata var. lannesiana ‘Shujaku' and C. serrulata var. lannesiana ‘Plena-pendula' possess two SCAR markers, C. jamasakura ‘Albo Plena' and C. serrulata var. lannesiana ‘Imose' possess three SCAR markers, C. subhirtella ‘Plena Rosea' and C.subhirtella‘Ujou-shidare' possess four SCAR markers, and C. serrulata var. lannesiana ‘Benigasa', C. serrulata var. lannesiana ‘Kouka', C.serrulata var. lannesiana ‘Hisakura', C. serrulata var. lannesiana ‘Contorta' and C. serrulata var. lannesiana‘Mollis'possess no SCAR markers. These SCAR markers can be used as cultivar-specific DNA fingerprints to promote the identification of 20 Cerasus cultivars, as well as privide molecular data for the establishment of identification system of Cerasus cultivars and for the further study of Cerasus germplasm.

CLC Number:

S685

ZHAO Qingjie, LI Haibo, QU Yan, WANG Qinghua, GUO Jia, XU Liang^{1 Symbolj@@}. SCAR markers-based molecular identification of 20 flowering cherries(Cerasus) cultivars[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2016, 59(05): 34-40.

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SCAR markers-based molecular identification of 20 flowering cherries(Cerasus) cultivars

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