Abstract: For early selecting excellent origin location of Phoebe bournei, we collected seeds from 17 Phoebe bournei origion locations in Guizhou, Zhejiang and Fujian Province and conducted container seedling test, to determine differences of the seedling height, ground diameter, root indices and biomass of both 1 and 2 years old Phoebe bournei seedlings. The results showed that there were significant difference in seedling height, ground diameter, total root length, total root surface area and biomass among different origin locations. The height, ground diameter, total root length, total root surface area and biomass of one year Phoebe bournei seedlings ranged respectively as follows:10.10-14.70 cm,2.02-2.93 mm, 55.57-133.22 cm, 10.92-30.90 cm²and 0.37-1.04 g. The height, diameter, total root length, total root surface area and biomass of two years Phoebe bournei seedlings ranged respectively as follows: 28.02-52.00 cm,4.00-5.05 mm, 189.00-299.44 cm,118.01-249.43 cm² and 3.66-14.13 g. By principal component comprehensive evaluation, we screened Jianou in Fujian, Lishui in Zhejiang, Chishui and Zunyi in Guizhou as the ideal origin location, they occupy better growth performance in Guiyang.