Identification of Bacillus pumilus LC01 and characterization of its spore laccase

doi:10.3969/j.issn.1000-2006.201609021

WANG Jiayi, LI Tailun, WANG Tiannü, LU Lei

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2018, Vol. 42 ›› Issue (05) : 113-120.

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JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2018, Vol. 42 ›› Issue (05) : 113-120. DOI: 10.3969/j.issn.1000-2006.201609021

Identification of Bacillus pumilus LC01 and characterization of its spore laccase

WANG Jiayi, LI Tailun, WANG Tiannü, LU Lei^*

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Abstract

【Objective】The previously isolated bacterial strain Bacillus pumilis LC01 displaying high laccase activity was presently investigated to determine spore laccase activity, with the goal of obtaining bacterial laccase with excellent properties for industrial applications. 【Method】LC01 was identified by morphology observation, physiological and biochemical tests, and 16S rDNA sequence analysis. The catalytic properties and dye degradation ability of the spore laccase were investigated under different conditions. 【Result】LC01 was identified as B. pumilus. The optimum pH of LC01 spore laccase was 3.0, 6.6 and 8.6 using 2,2'-2(3-ethylbenzothiazole-6-sulfonic acid), syringaldehyde, and 2,6-dimethoxyphenol as substrates, respectively. The spore laccase showed an optimal temperature of 80 ℃for syringaldazine oxidation. Activity was retained at a high level after incubation at pH 9.0 for 10 days or 50 ℃ for 10 h. The spore laccase-mediator system could efficiently decolorize different synthetic dyes. More than 90% of reactive black 5 and indigo carmine were decolorized within 1 h. Approximately 90% of remazol brilliant blue R was also decolorized after 6 h.【Conclusion】The pronounced efficiency of dye decolorization and good stability under alkali and high temperature conditions indicate the potential of the spore laccase of B. pumilus strain LC01 for diverse industrial applications.

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WANG Jiayi, LI Tailun, WANG Tiannü, LU Lei. Identification of Bacillus pumilus LC01 and characterization of its spore laccase[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2018, 42(05): 113-120 https://doi.org/10.3969/j.issn.1000-2006.201609021

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