Study on function of cathepsin bmcath1 in Bursaphelenchus mucronatus using method of RNA interference

doi:10.3969/j.issn.1000-2006.201612052

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2018, Vol. 42 ›› Issue (05): 65-70.doi: 10.3969/j.issn.1000-2006.201612052

Previous Articles Next Articles

Study on function of cathepsin bmcath1 in Bursaphelenchus mucronatus using method of RNA interference

PAN Yanyi^1,2, HUANG Lin¹, WU Xiaoqin^1*

1. Co-Innovation Center for the Sustainable Forestry in Southern China, Jiangsu Key Laboratory for Prevention and Management of Invasive Species, College of Forestry, Nanjing Forestry University, Nanjing 210037, China; 2. Huai'an Customs, Huai'an 223001,

Online:2018-09-15 Published:2018-09-15

Abstract

Abstract: 【Objective】This study aimed to analyze the function of cathepsin bmcath1 in Bursaphelenchus mucronatus.【Method】 Nematodes of B. mucronatus were treated with double-strand RNA(dsRNA)of bmcath1. Reproduction ability, feeding speed, sex ratio, body size, and pathogenicity were analyzed.【Result】Compared with the untreated B. mucronatus, the reproduction ability of nematodes treated by dsRNA of bmcath1 was increased by 75%. The dsRNA-treated nematodes also displayed a quicker feeding speed and stronger pathogenicity. The sex ratio of dsRNA-treated nematodes was changed from ♂3:♀5 to ♂6:♀13, and the percentage of adult nematodes increased from 8% to 19%.【Conclusion】Cathepsin bmcath1 has a significant effect on reproduction ability and pathogenicity in B. mucronatus.

CLC Number:

S763.1

PAN Yanyi, HUANG Lin, WU Xiaoqin. Study on function of cathepsin bmcath1 in Bursaphelenchus mucronatus using method of RNA interference[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2018, 42(05): 65-70.

References

[1] 董锦艳,李铷,张克勤.松材线虫生物防治研究进展[J].植物保护, 2005, 31(5): 9-15.DOI:10.3969/j.issn.0529-1542.2005.05.002.
DONG J Y, LI R, ZHANG K Q. Advances in biological control of Bursaphelenchus xylophilus by natural enemies and nematocidal compounds from plants[J]. Plant Protection, 2005, 31(5): 9-15.
[2] KIYOHARA T, BOLLA R. Pathogenic variability among populations of the pinewood nematode, Bursaphelenchus xylophilus[J]. Forest Science, 1990, 36(4): 1061-76.
[3] 张星耀,骆有庆.中国森林重大生物灾害[M].北京:中国林业出版社, 2004.
[4] WEBSTER J M, ANDERSON R V, BAILLIE D L, et al. DNA probes for differentiating isolates of the pinewood nematode species complex[J]. Revue de Nématologie, 1990, 13(3): 255-263.
[5] 汤坚, 叶建仁, 陈凤毛,等. 松树体内寄生线虫种类及其致病性[J]. 南京林业大学学报(自然科学版), 2008, 32(3): 112-116. DOI: 10.3969/j.issn.1000-2006.2008.03.027.
TANG J, YE J R, CHEN F M, SU S R, et al. Species of parasitic nematode in pine host and pathogenicity[J]. Journal of Nanjing Forestry University(Natural Sciences Edition), 2008, 32(3): 112-116.
[6] 张治宇,张克云,林茂松,等. 不同松材线虫群体对黑松的致病性测定[J]. 南京农业大学学报, 2002, 25(2): 43-46.
ZHANG Z Y, ZHANG K Y, LIN M S, et al. Pathogenicity determination of Bursaphelenchus xylophilus isolates to Pine thunbergii[J]. Journal of Nanjing Agricultural University,2002, 25(2): 43-46.
[7] 张治宇,林茂松,余本渊. 拟松材线虫对黑松苗的致病性[J].南京农业大学学报,2004,27(1): 46-50.
ZHANG Z Y, LIN M S, YU B Y. Pathogenicity of Bursaphelenchus mucronatus on the seedlings of black pine[J]. Journal of Nanjing Agricultural University,2004, 27(1): 46-50.
[8] 季宏铁.松材线虫(Bursaphelenchus xylophilus)与拟松材线虫(B. mucronatus)生物学杂交研究[D].南京:南京农业大学, 2004.
JI H T. Research of biological hybrid of Bursaphelenchus xylophilus and B. mucronatus[D]. Nanjing: Nanjing Agricultural University, 2004.
[9] 冯仕明,喻盛甫.拟松材线虫病危害不可忽视[J].云南林业, 2010(6): 29.
FENG S M, YU S F. Harm of Bursaphelenchus mucronatus is Assignable[J]. Yunnan Forest, 2010(6): 29.
[10] 陈凤毛,史延梅,王姝颖, 等. 不同株系拟松材线虫对黄山松和黑松苗木的致病性[J]. 林业科学, 2010, 46(12): 86-90. DOI:10.11707/j.1001-7488.20101214.
CHEN F M, SHI Y M, WANG S Y, et al. Pathogenicity of different isolates of Bursaphelenchus mucronatus to Pinus taiwanensis and P. thunbergii seedlings[J]. Scientia Silvae Sinicae, 2010, 46(12), 86-90.
[11] ZHOU L, CHEN F, PAN H, et al. Identifying virulence-associated genes using transcriptomic and proteomic association analyses of the plant parasitic nematode Bursaphelenchus mucronatus[J]. International Journal of Molecular Sciences, 2016, 17(9):1492. DOI: 10.3390/ijms17091492。
[12] KANG J S, LEE H, MOON I S, et al. Construction and characterization of subtractive stage-specific expressed sequence tag(EST)libraries of the pinewood nematode Bursaphelenchus xylophilus [J]. Genomics, 2009, 94(1): 70-77. DOI: 10.1016/j.ygeno.2009.03.001.
[13] 刘玉涛.松材线虫携带的细菌对其繁殖发育的影响[D].南京:南京林业大学,2003.
LIU Y T. The Effect of the bacteria carried by pine wood nematode Bursaphelenehus xylophilus on its reproduction and development[D]. Nanjing: Nanjing Forestry University, 2003.
[14] SINGH A, ROSENTHAL P J. Comparison of efficacies of cysteine protease inhibitors against five strains of Plasmodium falciparum[J]. Antimicrobial Agents and Chemotherapy, 2001, 45(3): 949-951. DOI: 10.1128/AAC.45.3.949-951.2001.
[15] WASILEWSKI M M, LIM K, PHILLIPS J, et al. Cysteine protease inhibitors block schistosome hemoglobin degradation in vitro and decrease worm burden and egg production in vivo[J]. Molecular and Biochemical Parasitology, 1996, 81(2): 179-189. DOI: 10.1016/0166-6851(96)02703-X.
[16] BERMAN J. Human leishmaniasis: clinical, diagnostic, and chemotherapeutic developments in the last 10 years[J]. Clinical Infectious Diseases, 1997, 24(4): 684-703.
[17] CARMONA C, DOWD A J, SMITH A M, et al. Cathepsin L proteinase secreted by Fasciola hepatica in vitro prevents antibody-mediated eosinophil attachment to newly excysted juveniles[J]. Molecular and Biochemical Parasitology, 1993, 62(1): 9-17. DOI: 10.1016/0166-6851(93)90172-T.
[18] GARCIA M P, NBREGA O T, TEIXEIR A R, et al. Characterisation of a Trypanosoma cruzi acidic 30 kDa cysteine protease[J]. Molecular and biochemical parasitology, 1998, 91(2): 263-272. DOI:10.1016/S0166-6851(97)00205-3.
[19] SOLANO S, CORTES I M, COPITIN N I, et al. Lymphocyte apoptosis in the inflammatory reaction around Taenia solium metacestodes in porcine cysticercosis[J]. Veterinary Parasitology, 2006, 140(1): 171-176. DOI: 10.1016/j.vetpar.2006.03.006.
[20] SHINGLES J, LILLEY C, ATKINSON H, et al. Meloidogyne incognita: molecular and biochemical characterisation of a cathepsin L cysteine proteinase and the effect on parasitism following RNAi[J]. Experimental Parasitology, 2007, 115(2): 114-120. DOI: 10.1016/j.exppara.2006.07.008.
[21] NEVEU C, ABAD P, CASTAGNONE-S P. Molecular cloning and characterization of an intestinal cathepsin L protease from the plant-parasitic nematode Meloidogyne incognita [J]. Physiological and Molecular Plant Pathology, 2003, 63(3): 159-165. DOI: 10.1016/j.pmpp.2003.10.005.

Related Articles 15

[1]	WEI Pengfei, LI Yongxia, FENG Yuqian, LIU Zhenkai, ZHANG Xingyao. Expression characteristics and biological functions of the sex determination gene Bx-sex-1 in Bursaphelenchus xylophilus [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2022, 46(4): 15-20.
[2]	JIANG Yi, CHEN Fengmao. Nematocidal activity of extracts of Alternanthera philoxeroides and Cayratia japonica against Bursaphelenchus xylophilus [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2022, 46(4): 45-52.
[3]	WU Jiawen, YIN Yannan, TAN Jiajin, HAO Dejun. A preliminary study on resistance of Pinus massoniana induced by Bacillus cereus NJSZ-13 strain to Bursaphelenchus xylophilus [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2022, 46(4): 53-58.
[4]	ZHANG Ruizhi, JIANG Shengwei, WU Hao, CHEN Qiaoli, LI Danlei, WANG Feng. Transcriptional activity of the Bx-HSF-1 in Bursaphelenchus xylophilus (Aphelenchida: Aphelenchoididae) [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2022, 46(4): 8-14.
[5]	YANG Yi, LIU Bo, YE Jianren, SU Luhui. A study on the ecological control of red blight of Metasequoia glyptostroboides by integrated forest management [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2021, 45(6): 90-98.
[6]	GAO Jingbin, XU Liuyi, YE Jianren. Growth and genetic diversity analysis of clones screened by phenotypical resistant to pine wilt disease in Pinus massoniana [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2021, 45(5): 109-118.
[7]	HE Qianqian, PENG Qi, ZONG Xiaozhuo, ZHU Tianhui, LI Shujiang. Induced resistance of Bambusa pervariabilis× Dendrocalamopsis grandis to Arthrinium phaeospermum [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2020, 44(5): 199-208.
[8]	SU Shengrong, ZHANG Qiaoqiao, YE Jianren. The pathogen and occurrence law of weeping willow leaf rust in the Huangshan Region [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2020, 44(4): 137-142.
[9]	SHANG Zheng, ZHOU Lifeng, FENG Yueyao, LIU Feiying, CHEN Fengmao. Ontogenesis of pine parasitic nematodeBursaphelenchus mucronatus (Nematoda: Aphelenchoididae) [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2019, 43(6): 11-17.
[10]	DING Sen, WANG Yan, LU Lanxiang, ZHANG Yuefeng, JIANG Mingming, WEI Qi, YE Jianren. Isolation, screening and identification of an endophytic bacteria in Ceresas with resistance to Agrobacterium tumefaciens and phosphorus solubilizing ability [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2019, 43(5): 81-88.
[11]	DAI Meili, FANG Lecheng, YIN Tongming, LI Xiaoping. Devleopment of SNP molecular markers against the foliar rust of poplar using SLAF-seq technique [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2019, 43(02): 73-78.
[12]	CHEN Feifei,HUANG Jinsi,WANG Xiyun,LIU Wanhui,YE Jianren. Cloning and expression analysis of polygalacturonase gene(BpPG) from Burkholderia pyrrocinia JK-SH007 [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2018, 42(04): 127-133.
[13]	LI Danlei, WANG Feng, CHEN Qiaoli, ZHANG Ruizhi, WANG Jianan. Impact of NDR1 gene on the incompatible Populus deltoides×P. trichocarpa infected with Melampsora larici-populina E4 [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2018, 42(01): 21-26.
[14]	ZHU Hanmingyue, PAN Yuanzhi. Screening for endophytic antagonistic bacterium of Jacaranda acutifolia and biocontrol effect against stem rot [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2017, 41(06): 61-67.
[15]	HU Zhongliang, ZHENG Cuiyun, TIAN Xingyi, LI Jingbao, HAN Zhengmin, FAN Ben. Determination of the inhibitory spectrum and stability of culture filtrate from Bacillus amyloliquefaciens HZM9 [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2017, 41(03): 65-70.

Metrics

Comments

www.nldxb.njfu.edu.cn

Edited ＆ Published by Editorial Department of Nanjing Forestry University(Natural Sciences Edition)
Address： No.159 Longpan Road,Nanjing 210037 Jiangsu,P.R.China
E-mail ：xuebao@njfu.edu.cn , xuebao@njfu.com.cn
Telephone +86-25-85428247,85427076
Distributed by China International Book Trading Corporation P.O. Box 399, Beijing ,P.R. China

Study on function of cathepsin bmcath1 in Bursaphelenchus mucronatus using method of RNA interference

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

Author

Reader

Reviewer