Study on function of cathepsin bmcath1 in Bursaphelenchus mucronatus using method of RNA interference

PAN Yanyi, HUANG Lin, WU Xiaoqin

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2018, Vol. 42 ›› Issue (05) : 65-70.

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南京林业大学学报（自然科学版）
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JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2018, Vol. 42 ›› Issue (05) : 65-70. DOI: 10.3969/j.issn.1000-2006.201612052

Study on function of cathepsin bmcath1 in Bursaphelenchus mucronatus using method of RNA interference

  • PAN Yanyi1,2, HUANG Lin1, WU Xiaoqin1*
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Abstract

【Objective】This study aimed to analyze the function of cathepsin bmcath1 in Bursaphelenchus mucronatus.【Method】 Nematodes of B. mucronatus were treated with double-strand RNA(dsRNA)of bmcath1. Reproduction ability, feeding speed, sex ratio, body size, and pathogenicity were analyzed.【Result】Compared with the untreated B. mucronatus, the reproduction ability of nematodes treated by dsRNA of bmcath1 was increased by 75%. The dsRNA-treated nematodes also displayed a quicker feeding speed and stronger pathogenicity. The sex ratio of dsRNA-treated nematodes was changed from ♂3:♀5 to ♂6:♀13, and the percentage of adult nematodes increased from 8% to 19%.【Conclusion】Cathepsin bmcath1 has a significant effect on reproduction ability and pathogenicity in B. mucronatus.

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PAN Yanyi, HUANG Lin, WU Xiaoqin. Study on function of cathepsin bmcath1 in Bursaphelenchus mucronatus using method of RNA interference[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2018, 42(05): 65-70 https://doi.org/10.3969/j.issn.1000-2006.201612052

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