Cloning and expression analysis of gene LiSEP3 in double lily

doi:10.3969/j.issn.1000-2006.2017.01.007

Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 2017, Vol. 41 ›› Issue (01) : 42-48.

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Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 2017, Vol. 41 ›› Issue (01) : 42-48. DOI: 10.3969/j.issn.1000-2006.2017.01.007

Cloning and expression analysis of gene LiSEP3 in double lily

Cloning and expression analysis of gene LiSEP3 in double lily

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Abstract

【Objetctive】 We cloned LiSEP3 from a double lily and analyzed LiSEP3 expression pattern among different aboveground organs and petals, for probing into SEP3 gene expression pattern in the double flower. 【Method】 LiSEP3 was isolated from double lily cv. ‘Belonica’ by RACE, and protein structure of LiSEP3 was predicted by SMART software. Phylogenetic analysis was performed by MEGA 5.0, subcellular localization of LiSEP3 were detected by particle bombardment. In addition, the expression pattern of LiSEP3 was analyzed among different aboveground organs and petals. 【Result】 LiSEP3 is a member of E class of MADS-box. The open reading frame of LiSEP3 is 729 bp in length, and encodes a protein of 242 amino acids. Protein structure analysis showed that LiSEP3 has a conserved MADS domain, a K-box region and two SEP motifs. Phylogenetic tree analysis suggested LiSEP3 had the highest similarity with monocotyledonous Alstroemeria. Subcellular localization assay revealed LiSEP3 was a nucleus protein. LiSEP3 was abundant in flowers while it couldn’t be detected in stems and leaves. LiSEP3 was expressed in petals from the first whorl to the seventh whorl, and had the highest transcription level in the seventh whorl petals. 【Conclusion】 LiSEP3 belongs to E class of MADS-box family genes and is specifically expressed in flowers and has a higher expression in seventh whorl than that in other whorls. Moreover, the expression pattern of LiSEP3 in flowers is different from its homologous genes in dicotyledons.

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Cloning and expression analysis of gene LiSEP3 in double lily. Cloning and expression analysis of gene LiSEP3 in double lily[J]. Journal of Nanjing Forestry University (Natural Sciences Edition）. 2017, 41(01): 42-48 https://doi.org/10.3969/j.issn.1000-2006.2017.01.007

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