JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2017, Vol. 60 ›› Issue (01): 199-202.doi: 10.3969/j.issn.1000-2006.2017.01.031

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Rearing techniques of Monochamus alternatus Hope(Coleoptera: Cerambycidae)on artificial diets

CHEN Ruixu1, WANG Lujie1, LIN Tao1, WEI Zhiqiang1, WANG Yan2, HAO Dejun1*   

  1. 1. Co-Innovation Center for the Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China;
    2. Forestry Station of Shanghai, Shanghai 200072, China
  • Online:2017-02-18 Published:2017-02-18

Abstract: 【Objetcive】 In order to acquire large number of even-aged and consistent physiological status larvae to provide enough insects for involved research, mass rearing techniques of Monochamus alternatus were carried out on artificial diets in the laboratory. 【Method】 Developmental time and activities at different stages were compared among A, B, C three kinds of artificial diets. A(pine sawdust 100 g, agar 40 g, sucrose 20 g, yeast extract 12.5 g, sodium benzoate 2 g, potassium sorbate 1 g, 0.5 mol/L H2SO4 10 mL, H20 800 mL), B(pine sawdust 100 g, agar 40 g, sucrose 20 g, yeast extract 12.5 g, sodium benzoate 2 g, potassium sorbate 1 g, 0.5 mol/L H2SO4 10 mL, cholesterol 1.5 g, ascorbic acid 4 g, casein 20 g, choline chloride 1 g, H20 800 mL), C(pine sawdust 100 g, agar 40 g, sucrose 20 g, yeast extract 12.5 g, sodium benzoate 2 g, potassium sorbate 1 g, 0.5 mol/L H2SO4 10 mL, wheat germ powder 25 g, cholesterol 1.5 g, ascorbic acid 4 g, casein 20 g, choline chloride 1 g, H20 800 mL). 【Result】 When provided with diet A, larval and pupal periods were(90.6±9.3)d and(11.9±1.3)d respectively, pupation rate was 6.4% and emergence rate was 66.7%. Pupal weights were(0.37±0.03)g for females and(0.30±0.04)g for males, and adult weights were(0.27±0.03)g for females and(0.21±0.02)g for males after 7 days emergence. When provided with diet B, larval and pupal periods were(71.9±4.0)d and(12.8±0.7)d respectively, pupation rate was 18% and emergence rate was 75.8%. Pupal weights were(0.33±0.02)g for females and(0.27±0.03)g for males, and adult weights were(0.29±0.02)g for females and(0.22±0.03)g for males after 7 days emergence. When provided with diet C, larval and pupal periods were(71.25±2.01)d and(11.12±0.34)d respectively, pupation rate was 58.33% and emergence rate was 88.10%. Pupal weights were(0.63±0.03)g for females and(0.49±0.03)g for males, and adult weights were(0.53±0.03)g for females and(0.43±0.03)g for males after 7 days emergence. The results showed that diet C was confirmed to be the optimal diet according to comparison of developmental time and activities among different modified diets. 【Conclusion】 It is suggested that diet C is superior to diet A and B, and can be a standard diet to laboratory population for further research work.

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