Effects of dimethyl sulphonate on proteome of Paulownia tomentosa seedlings infected by phytoplasma

doi:10.3969/j.issn.1000-2006.201801055

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2018, Vol. 42 ›› Issue (05): 77-83.doi: 10.3969/j.issn.1000-2006.201801055

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Effects of dimethyl sulphonate on proteome of Paulownia tomentosa seedlings infected by phytoplasma

DENG Minjie, JIN Ranran, ZHAO Zhenli, WANG Zhe, FAN Guoqiang^*

College of Forestry, Henan Agricultural University, Zhengzhou 450002, China

Online:2018-09-15 Published:2018-09-15

Abstract

Abstract: 【Objective】Determine the proteins related to Paulownia broom disease(PaWB)and provide a basis for studying its pathogenesis. 【Method】We used isobaric tags for relative and absolute quantitation(iTRAQ)to analyze proteomes for PaWB-infected Paulownia tomentosa seedlings treated and untreated with dimethyl sulphonate(DMS), and healthy seedlings as the control.【Result】A total of 2 628 proteins was identified, among which 177 proteins exhibited differential expression. By comparison, 37 differentially expressed proteins were found to relate to Paulownia witches' broom disease. Pathway analysis showed that these 37 proteins were mainly involved in photosynthesis, plant-pathogen interactions, and oxidative phosphorylation pathways. 【Conclusion】During the process of PaWB occurrence, the proteins related to photosynthesis were affected. CaM was involved in the interaction between phytoplasma and Paulownia. These results have important significance for the further study of the mechanism of Paulownia witches' broom disease at the protein level.

CLC Number:

S792.43

DENG Minjie, JIN Ranran, ZHAO Zhenli, WANG Zhe, FAN Guoqiang. Effects of dimethyl sulphonate on proteome of Paulownia tomentosa seedlings infected by phytoplasma[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2018, 42(05): 77-83.

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Effects of dimethyl sulphonate on proteome of Paulownia tomentosa seedlings infected by phytoplasma

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