Transcriptome analysis of differentially expressed genes in Reaumuria soongorica seeds germination under NaCl stress

LIU Xiaowei,YANG Xiuyan,WU Haiwen,LIU Xiaoyan,ZHU Jianfeng,ZHANG Huaxin

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2019, Vol. 43 ›› Issue (03) : 28-36.

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南京林业大学学报（自然科学版）
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JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2019, Vol. 43 ›› Issue (03) : 28-36. DOI: 10.3969/j.issn.1000-2006.201811020

Transcriptome analysis of differentially expressed genes in Reaumuria soongorica seeds germination under NaCl stress

  • LIU Xiaowei1,YANG Xiuyan1,WU Haiwen1,LIU Xiaoyan2,ZHU Jianfeng1*,ZHANG Huaxin1*
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Abstract

【Objective】Screen differentially expressed genes(DEG)at the germination stage of Reaumuria soongorica under NaCl stress and provide genetic resources for the breeding of salt-tolerant species.【Method】The R. soongorica seeds were treated with pre-fitting germination threshold concentration(273 mmol/L, marked as M), optimum germination concentration(43 mmol/L, marked as L)and distilled water(marked as C), and each treatment was repeated three times. The seeds were taken out when the radicle broke through the seed coat but not more than 2 mm, and the transcriptome sequencing was performed.【Result】There were 210, 2 273 and 2 888 DEG screened out in comparison group of L and C(LvsC), M and C(MvsC), M and L(MvsL), respectively, of which 116 were up-regulated and 94 were down-regulated in LvsC, 1 781 were up-regulated and 492 were down-regulated in MvsC, and 2 165 were up-regulated and 723 were down-regulated in MvsL. These up-regulated genes are mainly enriched in ribosome, carbon metabolism, cytochrome P450 metabolism, glutathione metabolism and other pathways in KEGG, down-regulated genes are mainly enriched in sucrose and starch metabolism, endoplasmic reticulum protein processing and other processes. 【Conclusion】Under stress conditions, the DEG induce related reactions synergistic responses, which ultimately lengthen the hypocotyl cells and break through the seed coat to complete germination.

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LIU Xiaowei,YANG Xiuyan,WU Haiwen,LIU Xiaoyan,ZHU Jianfeng,ZHANG Huaxin. Transcriptome analysis of differentially expressed genes in Reaumuria soongorica seeds germination under NaCl stress[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2019, 43(03): 28-36 https://doi.org/10.3969/j.issn.1000-2006.201811020

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