Pollen contamination and its influence on the second generation Eucalyptus pellita seed orchard

LI Peng, LUO Jianzhong, MO Jiyou, WANG Chubiao, LU Wanhong, LIN Yan

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2020, Vol. 44 ›› Issue (2) : 51-58.

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南京林业大学学报（自然科学版）
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JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2020, Vol. 44 ›› Issue (2) : 51-58. DOI: 10.3969/j.issn.1000-2006.201902018

Pollen contamination and its influence on the second generation Eucalyptus pellita seed orchard

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Abstract

【Objective】Pollen contamination in forest tree seed orchards can affect their gene purity and genetic structure. This study aimed to understand pollen contamination patterns and its influence on important traits in high-generation Eucalyptus pellita seed orchard, an exotic species, to provide a scientific basis for its germplasm conservation and long-term improvement. 【Method】Using 167E. pellita families from four seed orchards as genetic material, with leaf morphology as discriminating traits, this study analyzed 1.5-2.0 years trial data for pollen contamination rate and its distribution as well as its effects on plant growth, health and flowering phenology. 【Result】 Eucalyptus pellita from all four sources exhibited pollen contamination. Contamination rates were significantly different (P < 0.05) between sources, ranging from 18.75% to 35.50%. Family-level contamination rate was 92.81%. The contamination rates among families (within source) were highly significantly different ( P < 0.01). Pollen contamination highly significantly affected ( P < 0.01) leaf morphological traits, mainly leading to narrower leaves with a lighter color and thinner wax layer. However, it did not significantly affect leaf attitude. The effects of pollen contamination on the leaves were mostly significant among the four sources. Pollen contamination did not significantly affect tree volume growth and flowering phenology but significantly decreased tree health index by 4.74% ( P < 0.01).【Conclusion】Pollen contamination rate in the second-generation E. pellita was at a medium level. The results can be applied in modifying the methods of genetic conservation of E. pellita and preventing genetic contamination.

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Eucalyptus pellita / pollen contamination / high generation seed orchard / germplasm conservation

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LI Peng , LUO Jianzhong , MO Jiyou , et al . Pollen contamination and its influence on the second generation Eucalyptus pellita seed orchard[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2020, 44(2): 51-58 https://doi.org/10.3969/j.issn.1000-2006.201902018

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