JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 1993, Vol. 17 ›› Issue (01): 16-20.doi: 10.3969/j.jssn.1000-2006.1993.01.003

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THE X-RAY CONTRAST METHOD FOR TESTING GERMINABILITY OF CHINESE FIR SEEDS

Chen Yousheng Wu Qiongmei Chen Zhijian Gao Handong Shen Yongbao   

  1. The Southern Tree Seed Inspection Centre, Ministry of Forestry
  • Online:1993-03-18 Published:1993-02-18

Abstract: Potassium iodide was found in a pilot experiment to be the most suitable contrast agent a-mong 15 chemicals for Chinese fir seeds. It was confirmed that the best X-ray contrast method for Chinese fir seeds was as follows; Seed sample was soaked in tapwater for 16 hours at 25°C. After superficially drying, the sample was treated with a 40% aqueous solution of KI for 2 hours. Afterwards, the seeds were washed in slowly running water for 5-6 min. The extra water was wiped off the seeds. Thereafter, the seeds were dried at 70°C for 4 hours. The dried seeds were then radiographed under the following condition: kV=20, mA=3, exposure time=60s, focus-film distance=25cm. A seed with fully developed endosperm and embryo free of impregnation was considered as viable seed, Ger-minability could be thus calculated as ratio of the number of viable seeds to the total number of seeds in a sample. The germinabilities (X) of 34 Chinese fir seed lots evaluated by X-ray contrast method were very close to their germination percents (7) by the standard method. The relationship is Y=-1. 5 + 0. 998X(r=0. 97).