JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 1997, Vol. 21 ›› Issue (02): 50-53.doi: 10.3969/j.jssn.1000-2006.1997.02.011
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Ye Hanling Wang Chuanhuai John A.Buswell(Nanjing Forestry University Nanjing 210037)
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Abstract: Through systematical process for isolation and purification of laccase from fresh fruit body of Lenlinula edodes using the technique (NH4)2SO4 fractionation and ionexchange chromatography.We obtained one component of laccase,it showed homogeneity in the gel electrophorosis.The molecular weight was 55 000 by SDSPAGE,The optimum pH, temparature were 2.6 ℃ and 70 ℃，respectively. The studies of enzyme kinetics showed Km=0142 mmol
Ye Hanling Wang Chuanhuai John A.Buswell(Nanjing Forestry University Nanjing 210037). PURIFICATION AND PROPERTIES OF LACCASE FROM FRESH FRUIT BODY OF LENTINULA EDODES[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 1997, 21(02): 50-53.
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URL: http://nldxb.njfu.edu.cn/EN/10.3969/j.jssn.1000-2006.1997.02.011
http://nldxb.njfu.edu.cn/EN/Y1997/V21/I02/50
