PURIFICATION AND PROPERTIES OF LACCASE FROM FRESH FRUIT BODY OF LENTINULA EDODES

Ye Hanling Wang Chuanhuai John A.Buswell(Nanjing Forestry University Nanjing 210037)

Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 1997, Vol. 21 ›› Issue (02) : 50-53.

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南京林业大学学报（自然科学版）
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Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 1997, Vol. 21 ›› Issue (02) : 50-53. DOI: 10.3969/j.jssn.1000-2006.1997.02.011

PURIFICATION AND PROPERTIES OF LACCASE FROM FRESH FRUIT BODY OF LENTINULA EDODES

  • Ye Hanling Wang Chuanhuai John A.Buswell(Nanjing Forestry University Nanjing 210037)
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Through systematical process for isolation and purification of laccase from fresh fruit body of Lenlinula edodes using the technique (NH4)2SO4 fractionation and ionexchange chromatography.We obtained one component of laccase,it showed homogeneity in the gel electrophorosis.The molecular weight was 55　000 by SDSPAGE,The optimum pH, temparature were 2.6 ℃ and 70 ℃，respectively. The studies of enzyme kinetics showed Km=0142 mmol

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Ye Hanling Wang Chuanhuai John A.Buswell(Nanjing Forestry University Nanjing 210037). PURIFICATION AND PROPERTIES OF LACCASE FROM FRESH FRUIT BODY OF LENTINULA EDODES[J]. Journal of Nanjing Forestry University (Natural Sciences Edition）. 1997, 21(02): 50-53 https://doi.org/10.3969/j.jssn.1000-2006.1997.02.011
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