JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2000, Vol. 24 ›› Issue (02): 60-62.doi: 10.3969/j.jssn.1000-2006.2000.02.018

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Purification and Properties of Xylanase from Pseudomonas flu.

Su Yuping\ Wang Shuhan\ Ou Kai   

  1. College of Chemical Engineering Nanjing Forestry University\ Nanjing 210037
  • Online:2000-04-18 Published:2000-04-18

Abstract: Using the systematical process for isolation and purification of xylanase from Pseudomonas flu. with the tehnique (NH) 2SO 4 fractionation and Sephede x G100 gel column, we obtained the xylanase showing homogeneity in the gel electrophorosis.The melecular weight was 33?000 by SDS polyacrylamide.The optimum pH,temprature were 6.0 and 60?℃,respectively.The studies of enzyme kinetics showed K m=4.76?mg/mL.

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