Abstract: Based on the four month shading test of two year seedlings of ginkgo, some morphological, photosynthetic and physiological indexes were determined. The results showed: (1) there were significant differences in leaf area per seedling among different shading treatments, with a decreasing tendency from one layer net shading, to full sun light, to two layer net shading and to three layer net shading, (2) with the increase of shading intensity, the relative water content and chlorohyll content in leaves went up markedly, (3) as for carboxylation efficiency and CO 2 compensation point, there were no remarkable differences between the treatments of one layer net shading and full sun light. However, the carboxylation efficiency decreased and CO 2 compensation point increased remarkably in the treatments of two layer net shading and three layer net shading as compared with that in full illumination, (4) in terms of apparent quantum yields of photosynthesis, there were notable differences among different treatments, with a decreasing tendency from two layer net shading, to one layer net shading, to full sun light and to three layer net shading, and (5) with the increase of shading intensity, such indexes as fresh weight per leaf area, net photosynthetic rate, light saturation point and the dried leaves weight per seedling went down apparently.