JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2005, Vol. 29 ›› Issue (06): 7-10.doi: 10.3969/j.jssn.1000-2006.2005.06.002

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The Construction of Two’ Disease-resistance Gene NP1 and GAFP Vector

CHEN Ying1, DAI Yong-mei1,2, WANG Yi-qin3, SUN Yong-ru3, HUANG Min-ren1, WANG Ming-xiu1   

  1. 1. The Key Laboratory of Forest Tree Genetics and Engineering State Forestry Administration Nanjing Forestry University, Nanjing 210037, China; 2. Shanghai Flower Breeding Centre, Shanghai 200072, China; 3. Institute of Genetics and Development Biology, CAS, Beijing 100101, China
  • Online:2005-12-18 Published:2005-12-18

Abstract: Defensin is resistant to many kinds of virus, bacterium and fungusl. Gastrodia Antifungal Protein (GAFP) has the resistant ability to more than twenty kinds of fungi. The antidisease mechanisms and spectrum of the two genes are different. Using DNA reconstruction method a vector (pBin35SGAFPNP1) that contains two antidisease genes: NP1 and GAFP were constructed. Then two disease-resistance genes could be transferred into the same plant just through one time of transformation that can improve the disease-resistant ability of the transgenic plant to more kinds of pathogens. So the proceeding of plant antidisese breeding could be accelerated.

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