Construction, of cDNA Library from Stem Cambium Tissue of Cuninghamia lanceolata (Lam.) Hook

YANG Li-wei, SHI Ji-sen*

Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 2005, Vol. 29 ›› Issue (06) : 85-87.

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南京林业大学学报（自然科学版）
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Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 2005, Vol. 29 ›› Issue (06) : 85-87. DOI: 10.3969/j.jssn.1000-2006.2005.06.021

Construction, of cDNA Library from Stem Cambium Tissue of Cuninghamia lanceolata (Lam.) Hook

  • YANG Li-wei, SHI Ji-sen*
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Abstract

In order to isolate target genes for controlling the wood formation and quality components, a cDNA library was constructed from Cuninghamia lanceolata stems cambium tissue with the ZAP express phage vector. The host bacteria is E. coli XL1-Blue, and subclone bacteria is E. coli XLOLR. The compacity of primary library is 6.1 X l0s pfu, and the titer of amplified library is 5.2×109 pfu/mL, in which 95% of phages were recombinant, the size of fragments inserted rang from 0.5 kb to 3.0 kb.

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YANG Li-wei, SHI Ji-sen*. Construction, of cDNA Library from Stem Cambium Tissue of Cuninghamia lanceolata (Lam.) Hook[J]. Journal of Nanjing Forestry University (Natural Sciences Edition）. 2005, 29(06): 85-87 https://doi.org/10.3969/j.jssn.1000-2006.2005.06.021
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