JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2005, Vol. 29 ›› Issue (06): 119-122.doi: 10.3969/j.jssn.1000-2006.2005.06.030

Previous Articles     Next Articles

Cryopreservation of Chinese Pine Seeds

SHI Feng-hou, YU Fang-yuan*, SHEN Yong-bao, CAO Fu-liang   

  1. College of Forest Resources and Environment Nanjing Forestry University, Nan]ing 210037, China
  • Online:2005-12-18 Published:2005-12-18

Abstract: By investigating conductivity, dehydrogenase, germination percentage and germination index of cryopreserved Chinese pine seeds, feasibility of long-term storage of the seeds was studied. The results showed that: (1)the freezing and thawing was the main factor affecting the cryopreservation of the seeds; (2)cryoprotectant reduced the damage of the seeds cryopreserved,DMSO’s function was comparatively obvious; (3)the cryopreservation can be applied to store the Chinese pine seeds, and the optimal treatment combination was 10~DMSO+quick freezing+slow thawing.

CLC Number: