Malus prunifolia Borkh grows much faster than its homogeneities. Its flowers are provided with gorgeous characteristics, and possess great ornamental quality. The method of vegetative propagation was taken to produce seeding at present, In this article, the developmental course of the adventitious roots from cutting of M. prunifolia Borkh was observed with the anatomical method. The results showed as follows: (1) There was no incubate root primordium in cuttings of M. prunifolia Borkh, which was two years old. The induced root primordium developed into the adventitious root, and originated from the division and differentiation of the vascular cambium cell touching with the pith ray. After 15 days, adventitious root primordia developed into young adventitious roots. (2) The callus could not form an adventitous root primordium, and only form much sclerenehyma. The next step of the investigation will be stressed on the physiological mechanism of adventitious root from cutting of M. pruni folia.