JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2009, Vol. 33 ›› Issue (01): 0-.doi: 10.3969/j.jssn.1000-2006.2009.01.001

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Evaluation of antagonistic microorganisms for biocontrol of Botrytis cinerea in cyclamen

LI Dewei,John C Sutton   

  1. Department of Environmental Biology,University of Guelph,Guelph,ON,N1G 2W1,Canada
  • Online:2009-02-18 Published:2009-02-18

Abstract: In biocontrol essays on cyclamen leaf disks,Clonostachys rosea and Trichoderma koningii suppressed Botrytis cinerea by 94%—100%,and were more effective than most fungi and bacteria isolated from cyclamen.Biocontrol effectiveness on whole plants showed that C.rosea suppressed B.cinerea in leaves,petals,petiole,and pedicels by 96%,58%,53%,and 77%,respectively and T.koningii by 100%,42%,100% and 90%,respectively.The effects of inoculum concentrations of B.cinerea interacting with C.rosea or T.koningii respectively on biocontrol were examined in factorial design experiments.C.rosea suppressed B.cinerea in lamina,petals,petiole,and pedicels by 73%—100%,52%—86%,100% and 54%—68%,respectively,when inoculum concentration of the antagonist was 10 times more than that of the pathogen.When inoculum concentration of T.koningii was at or over 106 conidia/mL,it suppressed B.cinerea at concentrations of 103—106conidia/mL in lamina by 100%,in petioles by 62%—100%,in petal by 38%—96% and in pedicels by 56%—100%.C.rosea was more effectively on 2,3,5 and 6 weekold leaves,but less effective on 4 weekold leaves and no effect on 1 weekold leaves.T.koningii suppressed B.cinerea on 1 to 6 weekold leaves very effectively ranging from 89% to 100%.We concluded that C.rosea and T.koningii were potentially effective biocontrol agents against B.cinerea in cyclamen in greenhouses,but the effectiveness of T.koningii was significantly better than that of C.rosea.

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