Using MuPS marker technique to identify individual of Pinus massoniana resistance to pine wilt disease

doi:10.3969/j.jssn.1000-2006.2009.03.001

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2009, Vol. 33 ›› Issue (03): 1-4.doi: 10.3969/j.jssn.1000-2006.2009.03.001

Using MuPS marker technique to identify individual of Pinus massoniana resistance to pine wilt disease

GAO Jingbin1,2, XU Liuyi2, YE Jianren1*, ISODA Keiya3, UBUKATA Masatoshi3

1.College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China; 2.Anhui Province Forestry Bureau Resistance Breeding for Pine nematode Centre, Hefei 230031, China; 3.Forest Tree Breeding Center Forestry Research Products Research Institute, Ibaraki 3191301, Japan

Online:2009-06-18 Published:2009-06-18

Abstract

Abstract: Using 14 SCAR marker primers to analyze the resistance seedlings of Pinus massoniana which resist Bursaphelenchus xylophilus. The results indicated that there were 104 different types of MuPS among those 120 samples, which could distinguish 86.6% samples, and 74.2% samples identified completely. Some primers such as Pmscar157, Pmscar007 and Pmscar053 produced more bands, which was 13.1%, 13.3%, 18.4% of the total bands respectively, while the primers Pmscar039 and Pmscar499 produced less band, and they were 0.16% and 0.48% of the total bands respectively. The primers used in this paper could fingerprint well because of high stability and reliability. Therefore, the attained MuPS could be used to identify and manage the resistance individual and the resistance family of P.massoniana.

CLC Number:

S763

GAO Jingbin1,2, XU Liuyi2, YE Jianren1*, ISODA Keiya3, UBUKATA Masatoshi3. Using MuPS marker technique to identify individual of Pinus massoniana resistance to pine wilt disease[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2009, 33(03): 1-4.

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Using MuPS marker technique to identify individual of Pinus massoniana resistance to pine wilt disease

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