In order to select strong coldresistant tree species, seedlings of six tropical and subtropical species (Dillenia turbinata, Peltophorum tonkinense, Garcinia oblongifolia, Alstonia scholaris, Khaya senegalensis and Ormosia pinnata) were treated with the lasting times of 0.5, 3, 5 and 8 d at 10℃ respectively. The changes of physiological indices of these seedling were studied. The results showed that with low temperature stress time increasing, the relative electric conductivity of six seedlings tended to increase, the contents of free proline fluctuated rapidly, and the chlorophyll contents fluctuated slightly, whereas the contents of MDA generally increased and then decreased. Except for D.turbinata, the activities of SOD were closed to the controls after fluctuation. With increasing low temperature, the contents of soluble protein of six tree species were greater than or closed to the controls after fluctuation. Cold resistance of six seedlings was evaluated using principal component analysis and the their cold resistance was O.pinnata, K.senegalensis, A.scholaris, G.oblongifolia, P.tonkinense, D.turbinate.
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
References
[1]巫光宏,詹福建,黄卓烈,等.低温胁迫对马占相思树代谢的影响研究[J]. 林业科学研究,2001,14(6):633-640.
[2]孙清鹏,许煌灿,张方秋,等.低温胁迫对大叶相思和马占相思某些生理特性的影响[J] . 林业科学研究,2002,15(1):34-40.
[3]柴团耀,张玉秀. 菜豆富含脯氨酸、蛋白质基因在生物和非生物胁迫下的表达[J] . 植物学报,1999,41(1):111-113.
[4]余叔文,汤章诚. 植物生理与分子生物学[M] . 北京:科学出版社,1998.
[5]徐燕,薛立,屈明.植物抗寒生理生态机理研究进展[J]. 林业科学,2007,43(4):88-94.
[6]Crawford R M. Studies in Plant Survival Ecological Case Histories of Plant Adaptation to Adversity[M]. Melbourne: Blackwell Scientific Publication, 1989.
[7]陈建勋,王晓峰. 植物生理学实验指导[M]. 广州:华南理工大学出版社,2002.
[8]李合生,孙群, 赵世杰,等. 植物生理生化实验原理和技术[M]. 北京:高等教育出版社,2000.
[9]Bradford M M A. Rapid and sensitive method for the quantation of microgram quantities of protein utilizing the principle of proteindye binding[J]. Anal Biochem, 1976, 72: 248-254.
[10]区靖祥,邱健德. 多元数据的统计分析方法[M]. 北京:中国农业科学技术出版社,2002.
[11]王青宁,唐静,衣学慧. 基于多元统计评价毛白杨无性系的抗旱性[J]. 西北林学院学报,2005,20(4):21-26.
[12]张圣平,顾兴芳,王烨,等. 低温胁迫对以野生黄瓜(棘瓜)为砧木的黄瓜嫁接苗生理生化指标的影响[J]. 西北植物学报,2005,25(7):1428-1432.
[13]汤章诚. 逆境条件下植物脯氨酸的累积及可能意义[J]. 植物生理学通讯,1984(1):15-27.
[14]冯建灿,张玉洁,杨天柱. 低温胁迫对喜树幼苗SOD活性、MDA和脯氨酸含量的影响[J]. 林业科学研究,2002,15(2):197-202.
[15]杨德浩,杨敏生,王进茂,等. 欧洲白桦苗期低温胁迫时膜系统的变化[J]. 东北林业大学学报,2004,32(6):13-15.
[16]刘慧英,朱祝军,吕国华. 低温胁迫对嫁接西瓜耐冷性和活性氧清除系统的影响[J]. 应用生态学报,2004,15(4):659-662.
[17]马德华,卢育华,庞金安. 低温对黄瓜幼苗膜脂过氧化的影响[J]. 园艺学报,1998,25(1):61-64.
[18]吕成群,黄宝灵. 低温胁迫对巨尾桉幼苗膜脂过氧化及保护酶的影响[J]. 广西植物,2004,24(1):64-68.
PDF(847353 KB)
